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Planning rice breeding programs for impact Pedigree Selection in the IRRI Irrigated Breeding Program Parminder S. Virk PBGB Division.

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Presentation on theme: "Planning rice breeding programs for impact Pedigree Selection in the IRRI Irrigated Breeding Program Parminder S. Virk PBGB Division."— Presentation transcript:

1 Planning rice breeding programs for impact Pedigree Selection in the IRRI Irrigated Breeding Program Parminder S. Virk PBGB Division

2 IRRI: Planning Breeding Programs for Impact Can anyone define “pedigree”?

3 IRRI: Planning Breeding Programs for Impact Describe the pedigree method Describe naming of pedigree at IRRI Describe crosses at IRRI Describe types of crosses Demonstrate IRRI nurseries Learning objectives

4 IRRI: Planning Breeding Programs for Impact  The pedigree method owes its name to the pedigree record of selected plants maintained to trace ancestral relationship among such plants A detailed description of present day pedigree method was outlined by Love (1927) Pedigree Method

5 IRRI: Planning Breeding Programs for Impact In this method individual plants are selected starting with F 2 and in the subsequent segregating generations their progenies are tested till the progenies become homozygous. During entire operation a record of all parent-offspring relationship is kept: PEDIGREE RECORD In this method each progeny in every generation can be traced back to the original F 2 plant Pedigree describes parents, grandparents, great-grandparents,... Pedigree Method

6 IRRI: Planning Breeding Programs for Impact Based on cross number and serial numbers of selected plants  IR numbers are assigned consecutively to the crosses (now IR80389) made at IRRI just before the hybrid seeds are incubated for germination  When plant selections are made from F 2 and subsequent generations of an IR cross, numerical designations are given for each breeding line How is the naming of pedigree at IRRI?

7 IRRI: Planning Breeding Programs for Impact Selections from the F 2 were grown in the pedigree nursery as F 3 rows: IR2061-1, ------IR2061-214 -----and so on up to IR2061-806 From selection 214, three plants were grown as F 4 selections: IR2061-214-1, IR2061-214-2 and IR2061-214-3 From selection IR2061-214-3, 10 plants were again selected as F 5 rows These selections were designated as IR2061-214-3-1 up to 2061-214-3- 10 From selection IR2061-214-3-8, three plant selections were made and planted in the pedigree nursery as F 6 rows IR2061-214-3-8-1, IR2061- 214-3-8-2 and IR2061-214-3-8-3 At maturity selection IR2061-214-3-8-2 appeared uniform and was bulk harvested and evaluated in replicated trials Example: IR2061-214-3-8-2 (IR28)

8 IRRI: Planning Breeding Programs for Impact How is the naming of pedigree in your country/institute?

9 IRRI: Planning Breeding Programs for Impact Procedure What are the breeding objectives? High Yield Potential Short Growth Duration Superior Grain Quality Multiple disease and insect resistance Choice of Parents?

10 IRRI: Planning Breeding Programs for Impact  Staggered sown 3 times each season  at 2-weeks interval IRRI Varieties are donors in various programs as  Irrigated  Rainfed  Flood prone and  Hybrid rice Donors for various traits (Hybridization Block)

11 IRRI: Planning Breeding Programs for Impact Hybridization Block

12 IRRI: Planning Breeding Programs for Impact Hybridization Block

13 IRRI: Planning Breeding Programs for Impact Field Book

14 IRRI: Planning Breeding Programs for Impact Summary of HBs Electronic Field Book

15 IRRI: Planning Breeding Programs for Impact Selected Donors

16 IRRI: Planning Breeding Programs for Impact Planning Crosses!

17 IRRI: Planning Breeding Programs for Impact  For Yield, grain quality and incorporation of resistance to diseases and insects Released varieties x Elite Indica Lines Elite Indica Lines x Elite Indica Lines Elite Indica Lines x Basmati Type Lines Further improvement in Indica germplasm

18 IRRI: Planning Breeding Programs for Impact Basmati x Elite Indica Lines Basmati x Basmati Type Derivatives Basmati Type Derivatives x Basmati Type Derivatives Developing Basmati Type Germplasm

19 IRRI: Planning Breeding Programs for Impact Tropical Japonica x Tropical Japonica crosses Tropical Japonica x Korean Varieties Improving Tropical Japonicas for temperate regions (Korea)

20 IRRI: Planning Breeding Programs for Impact  For Yield, grain quality and incorporation of resistance to diseases and insects Improved NPTs x Improved NPTs Improved NPTs x Released Indica Varieties Improved NPTs x Elite Indica Varieties Further Improvement in NPTs

21 IRRI: Planning Breeding Programs for Impact What crosses do you currently focus on in your institute?

22 IRRI: Planning Breeding Programs for Impact Types of crosses:  Single crosses  Backcrosses  Three way/Top crosses  Around 900 crosses yearly (wet & dry season) Cross designation (IR80369) F1 Nursery

23 IRRI: Planning Breeding Programs for Impact

24 F1

25 IRRI: Planning Breeding Programs for Impact  Grow 2-6 rows (30 Plants) /cross  Rouge off-types  Identify accidental selfs Field Operations

26 IRRI: Planning Breeding Programs for Impact Protected:  In general susceptible to diseases and insects Unprotected:  In general resistant to diseases and insects First grow in blast nursery & Reject Susceptible populations Space planted 35 x 25 cm. Population size from 2,500- 5,000 individuals F2 Nursery

27 IRRI: Planning Breeding Programs for Impact Blast Nursery

28 IRRI: Planning Breeding Programs for Impact Blast Nursery

29 IRRI: Planning Breeding Programs for Impact Blast Nursery

30 IRRI: Planning Breeding Programs for Impact Protected F2

31 IRRI: Planning Breeding Programs for Impact Unprotected F2

32 IRRI: Planning Breeding Programs for Impact Inoculate with BB Race1/Race2 Rogue susceptible/undesirable plants Harvest desirable plants (100-500 plants) Field Operations

33 IRRI: Planning Breeding Programs for Impact Four nurseries/year: January & July (Unprotected) May & November (Protected)  Space planted 30 x 20 cm  Every 20 th row is a check variety (4 Checks)  16,000 to 20,000 rows (30 plants) Pedigree nursery What system do you have in your institute?

34 IRRI: Planning Breeding Programs for Impact Pedigree Nursery in May

35 IRRI: Planning Breeding Programs for Impact Pedigree Nursery in May

36 IRRI: Planning Breeding Programs for Impact Pedigree Nursery in July

37 IRRI: Planning Breeding Programs for Impact Pedigree Nursery in July

38 IRRI: Planning Breeding Programs for Impact Screening requirements Memo

39 IRRI: Planning Breeding Programs for Impact Field Book

40 IRRI: Planning Breeding Programs for Impact  F3 single rows  F4 onwards 3/6 rows  Selection between rows/within rows- at least 3 plants.

41 IRRI: Planning Breeding Programs for Impact 17811 Progeny Rows Composition of 2004 NOV PN GenCross typeNo. GenCross typeNo. F3I/I/I4 F5I/I5 IND/IND18 I/I/I2 IND/WH3 I/J/I18 JAP/BAS/ARO4 J/I6 JAP/I/I1 J/J3 Jap/Korean10 Micronutrient21 NPT/IND19 F6I/I17 NPT/JAP/IND3 NPT/I32 F4I/I11 NPT/NPT17 I/I/I1 J/I/I18 I/J31 Micronutrient40 NPT/BAS6 F7I/J2 NPT/I/I5 Micronutrient20 NPT/NPT14 NPT/NPT/I3

42 IRRI: Planning Breeding Programs for Impact CrossParentsStartEnd Sel. Gen. Type IR 79228IR 72903-6-2-2-146001460066F7I/J IR 67966-44-2-3-2 IR 79232IR 72905-49-3-2-246007460093F7I/J IR 68552-100-1-2-2 IR 79483IR 71700-247-1-1-246010460156F6I-NPT/NPT IR 68544-29-2-1-3-1-2 IR 79484IR 71700-247-1-1-246016460183F6I-NPT/NPT IR 72225-20-3-2-3 IR 79485IR 71700-247-1-1-2460194603718F6I-NPT/NPT IR 73707-45-3-2-3 IR 79487IR 72158-11-5-2-3460384605012F6I-NPT/NPT IR 72225-20-3-2-3 IR 79489IR 72967-12-2-346051460533F6I-NPT/NPT IR 68544-29-2-1-3-1-2 IR 79493IR 67961-15-1-1460544607218F6NPT/NPT IR 68544-29-2-1-3-1-2 IR 79494IR 67961-15-1-146073460829F6NPT/NPT IR 72225-29-1-2-1 2004 NOV PN

43 IRRI: Planning Breeding Programs for Impact OYT

44 IRRI: Planning Breeding Programs for Impact RYT

45 IRRI: Planning Breeding Programs for Impact Any questions or comments?

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