1. Tree evaluation on station Tony Simons, ICRAF, Kenya

5. Tree evaluation on station 1.Objectives of evaluation 2.Advantages of work on station 3.Disadvantages of work on station 4.Types of trials

6. 1. Objectives of evaluation Trials cost in terms of both time and money, so: Why is the trial needed? What hypothesis are you testing? How many treatments do I need/have? What do you plan to measure? How often? Has anyone else researched this before? (CSIRO, CIRAD, CATIE, EMBRAPA, DFSC, OFI, ASEAN, Winrock) How long is the trial envisaged to last? What will the trial lead to? Can it be done satisfactorily on farm? Are you trying to get too much/too little from it?

7. 2. Advantages of work on station Ease of access, more frequent monitoring Nursery is usually closer, planting done quicker Better control of the conditions (water, weeds, etc) Need for fewer replicates as less variable site Better security (theft, interference, fire) Fewer constraints on what is permissible Gain understanding before going on farm Trials can be larger and/or more complicated Visitors can see many trials in one place Often historical records (field and climate) May have a conservation role (don’t over play)

8. 3. Disadvantages of work on station May be unrepresentative of farmers’ conditions - lead to false conclusions for on farm work - farmers don’t relate to it - the control treatment may be misleading Can be expensive to maintain Researchers can be reluctant to close trials Default time fillers for labourers

9. 4. Types of trials A. Species trials B. Species/provenance trials C. Provenance tests D. Provenance/family trials E. Family (progeny) tests F. Clonal trials G. Management trials

10. A. Species trials Four kinds - Elimination (> 10 species, arboreta) - Comparative (2-10 species) - Proving trials (1-2 species) - Modelling (e.g. WaNuLCaS) What is the species required for? How many species are available? What is likely to grow well? (species-site matching) Exotic/indigenous? Does the seedlot represent the species? (# prov.) Careful if species have contrasting growth

11. A. Species trials (cont.) Appropriate plot designs: (usually with border trees/rows) - blocks (square, rectangular) - lines Plant at final spacing or thin? (50% diagonally) What is the control? Beware of confounding (species x management) Problems arise if survival is moderate to low Need sufficient number of trees (>50 trees) Although often suggested, few species mixtures Encourage correct botanic nomenclature Biosafety considerations (weediness, quarantine) What is the species required for?

12. Square plots (measured trees/total) 3 x 3 (1/9) 4 x 4 (4/16) 5 x 5 (9/25) 6 x 6 (16/36) 7 x 7 (25/49) 8 x 8 (36/64)

13. Prov. AProv. BProv. C Calliandra 407090 Gliricidia 6011070 Leucaena 1008060 Fodder yield per plot (kg) at 18 months, Embu, Kenya

14. Prov. A Calliandra 40 Gliricidia 60 Leucaena 100 Diaminatou’s trial

15. Prov. B Calliandra 70 Gliricidia 110 Leucaena 80 Patrick’s trial

16. Prov. C Calliandra 90 Gliricidia 70 Leucaena 60 Telesphore’s trial

17. Prov. AProv. BProv. C Calliandra 407090 Gliricidia 6011070 Leucaena 1008060 Fodder yield per plot (kg) at 18 months, Embu, Kenya

18. Fruit yield (kg) 36.8 40.1 Species A Species B

19. Fruit yield (kg) 36.8 40.1 Species A Species B

20. B. Species/provenance trials Several provenances tested per species Objective: (a) to identify best species (b) to identify best provenance (s,xs) Not necessary to have equal numbers of provenances per species, but if unbalanced be cautious with species comparisons Design and analysis is more complex What is the species required for?

21. C. Provenance tests expect 2-5 fold differences between provenances ensure seedlot has broad genetic base (>30 parent trees) depending on objectives and species, then need 100-400 trees is the material well documented? can you get more seed if it is needed? do you plan to convert the trial to a seed stand? where most G x E tests are done (interpret/use?) to date few done on farm, more could be

22. D. Provenance/family trials Several families tested per provenance Objective: (a) to identify best provenances (b) to identify best families (p,xp) (c) to calculate genetic parameters Not necessary to have equal numbers of families per provenance, but if unbalanced be cautious with provenance comparisons Require >30 families per provenance Design and analysis is most complex What is the species required for?

23. E. Family (progeny) tests Used for calculating genetic parameters (s.e.) - these are age, site, population, trait specific Used to identify best families (backward seln - cso) Used to identify next parents (forward seln) Used for phenology studies, breeding system Can be full-sib (we know mother and father) or half-sib (we know only mother) Require >30 families, many more for family seln Generally require >20 trees per family What is the species required for?

24. Family 01 0.6 11 0.8 21 1.3 02 1.2 12 1.3 22 0.7 03 0.7 13 0.9 23 0.5 04 1.3 14 0.7 24 1.3 05 0.5 15 1.4 25 0.6 06 0.7 16 1.4 26 1.5 07 1.2 17 0.8 27 0.9 08 1.1 18 1.1 28 1.3 09 1.5 19 1.2 29 1.4 10 0.6 20 0.7 30 0.8 Overall mean = 1.0 Height (m) at 12 months for 30 families of Prunus africana

25. Family 01 0.6 11 0.8 21 1.3 02 1.2 12 1.3 22 0.7 03 0.7 13 0.9 23 0.5 04 1.3 14 0.7 24 1.3 05 0.5 15 1.4 25 0.6 06 0.7 16 1.4 26 1.5 07 1.2 17 0.8 27 0.9 08 1.1 18 1.1 28 1.3 09 1.5 19 1.2 29 1.4 10 0.6 20 0.7 30 0.8 Overall mean = 1.0, mean of three = 1.4 Height (m) at 12 months for 3 (of 30) families of Prunus africana

26. F. Clonal trials To observe clonal differences for selection To determine clonal repeatability To determine any “c” effects Can be used for clonal seed orchards, if rogue Can be used to set up mother blocks, if rogue Good for mating system experiments What is the species required for?

27. G. Management trials careful to ensure relevance to on-farm conditions can investigate individual factors and interactions: - spacing - thinning - watering - pruning - fertilising - shading - microsymbionts - topworking, grafting, budding - nursery carry-over experiments

28. Most published tree species and provenance trials are for commercial forestry species Caution needed in AF with: - different designs - replication - farmer’s objectives …especially if extrapolating from a temperate trial

30. If you are going to do advanced improvement then don’t shortcut trials Get your species right Get your founder material right

31. It is desirable if you can carry your blocking through from the nursery to the field.

32. Trials can be converted to seed stands, … composition, design, layout, numbers, management