1. Review 3 C483 Spring 2013

2. Net outcomes of Oxidative phosphorylation (shuttles) Fatty acid catabolism Nitrogen catabolism Light reactions of photosynthesis Calvin cycle

3. Structures to know All amino acids (deoxy)ribonucleosides and (deoxy)ribonucleotides Carbamoyl phosphate and urea Pyruvate, oxaloacetate,  -ketoglutarate PRPP

4. Metabolic Pathways/Reactions Know these reactions/pathways (not arrow mechanisms) – Beta oxidation – PLP-mediated transamination – Formation of carbamoyl phosphate – Catabolism of amino acids covered in lecture – Basic hydrolysis and RNase-catalyzed hydrolysis of RNA

6. Protonmotive Force NADH + H + + ½ O 2  NAD + + H 2 O + 10 H + pumped succinate + ½ O 2  fumarate + H 2 O + 6 H + pumped

20. Photosynthesis: Net Reaction 2 H 2 O + 4 NADP + + 8 photons  O 2 + 4 NADPH

21. Proton Gradient For every ONE O 2 – 4 e - passed – 8 photons absorb – 12 H + pumped In plants, 12 H + required to turn ATP synthase – 3 ATP made Quantum yield is 3 ATP/8 photons or ATP/2.7 photon