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Published byGwenda Higgins Modified over 9 years ago
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1.Medical Purposes 2.Reviving endangered species and extinct species 3.Reproducing a deceased pet 4.Cloning humans?????
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Mysteries that can be solved by DNA Crimes (murder, rape, robbery, etc.) Human Identification Prove Kinship (paternity, maternity, etc.. Prove innocence Genetic disorders Historical mysteries Evolution trends
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By comparing DNA fingerprints of the unknown to the known Samples are created by gel electrophoresis
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also called DNA testing, DNA typing, or DNA profiling is a technique used to distinguish between individuals of the same species using only samples of their DNA
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Separat e DNA Isolate DNA Using a lysis solution and a warm water bath Using an ultracentrifuge (separated based on density) Using forms of alcohol to clump DNA Process of removing DNA from cells received from samples of blood, skin, semen, etc.
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piece of equipment, generally driven by a motor, that puts an object in rotation around a fixed axis The centrifuge works using the sedimentation principle where the centripetal acceleration is used to separate substances of greater and lesser density
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Process in which DNA is “cut” by restriction enzymes into fragments Restriction Enzymes are enzymes that cut DNA at SPECIFIC sequences of nucleotides.
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is a technique used for the separation of DNA, RNA, or protein molecules using an electric current applied to a gel matrix, based on their size and electric charge Gel electrophoresis gel animation
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To determine differences in the genomes of different plant and animal species The number and positions of the bands formed on each lane of gel is the actual DNA fingerprint and is unique to each person
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DNA EXTRACTION DNA FRAGMENTATION GEL ELECTROPHORESIS
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Using your notes and your knowledge of DNA fingerprinting, make a list of materials and tools needed for us to perform a paternity test? http://www.youtube.com/watch?v=-NMHGkOBXic
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Lysis solution DNA samples ultracentrifuge Restriction enzymes Gel chamber Power supply paternity test Alcohol(s) Warm water bath
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