1. Dr H Schnerr Leatherhead Food International Ltd., Randalls Road, Leatherhead, Surrey, KT22 7RY  +44 (0) 1372 822203  hschnerr@leatherheadfood.com  www.leatherheadfood.com Biosensor Technology An Innovative Option for the Food Sector

2. SPR Suppliers CompanyInstrumentWebsite Biacore http://www.biacore.com Affinity SensorsIAsyshttp://www.affinity-sensors.com IBIS TechnologiesIBIShttp://www.ibis-spr.nl Texas InstrumentsSPREETAhttp://www.ti.com Analytical µ-Systems BIOSUPLARhttp://www.biosuplar.de GWC technologiesSPRimagerhttp://www.gwctechnologies.com ReichertRefractometer- SR7000 http://www.reichertai.com INIXhttp://www.inex.org.uk

3. Objectives of the Experiment Concentration Analysis: How MUCH? Active Concentration Solution Equilibrium Inhibition Affinity Analysis: How STRONG? K D, K A Relative Ranking Yes/No Data Ligand Fishing Kinetic Rate Analysis: How FAST? k a, k d K D = k d /k a, K A = k a /k d

4. SPR technology Label-free Real-time Unique, high quality data on molecular interactions Simple assay design Robust and reproducible Walk-away automation Minimal sample preparation Rapid method

5. Binding Event Real-time Sensorgram Biomolecular Binding in Real Time

6. Principle of Detection  1   RU Time

7. Direct measurement Indirect measurement Inhibition in solution assay (ISA) Surface competition assay (SCA) Direct Binding Assay (DBA) Flexibility in Assay Design

8. Assays Relevant to Industry Food Quality Vitamins Biotin Folic Acid B 12 Pantophenic Acid (B 5 ) Authenticity Nonmilk Protein in Milk Powder GMO Testing Process-dependent Food Change Pasteurisation of Milk

9. Assays Relevant to Industry Food Safety Mycotoxins DON Aflatoxin Antibiotics, growth promoters and veterinary drug residues Streptomycin Chloramphenicol  -agonists Food Pathogens Salmonella E. coli O157:H7

10. Assays Relevant to Industry Food Related Studies Protein-Polysaccharide-Interactions  -casein and  -/  -carrageenan Binding Interaction Studies of Food Pathogens Salmonella and Collagen/Muscle Proteins E. coli O157:H7 and Collagen Interactions with membranes, receptors etc. Looking for Bioactive Appetite Suppressants

11. Assays Relevant to Industry Life Science/Drug Discovery Proteomics Ligand Fishing SPR/MS Antibody Characterisation Isotyping Affinity Kinethic Target ID Elucidate Disease Mechanisms Define Drugs or Diagnostic Markers

12. Sensor Development - Biotin/Folic Acid Pathogen Detection - Salmonella Allergen Testing Interaction studies - Polysaccharide - Protein Method Evaluation & Validation Routine Analysis - Qflex ® Kits for Vitamins LFI and Biosensors

13. SPR detects binding events as changes in mass at the chip surface Real-time kinetic measurements Label-free technology Qualitative rankings Flexibility in assay design Minimal sample preparation One platform for many different assays Summary

14. Biosensor Technology An Innovative Option for the Food Sector