1. What is in a scientific article plus DNA Structure and Function Bonus #1 is due 10/02/08. Bonus #2 is due 11/25/08.

2. Scientists communicate by publishing their findings in journals…

3. What is in a journal article?

5. Basic info: who, what, where, when

6. The abstract is a summary of the rationale and results.

7. The introduction has background information.

8. What is this?

9. References are how scientists cite other people’s ideas or data. (Castrogiovanni et al., 1998) Castrogiovanni P, Iapichino S, Pacchierotti C, Pieraccini F. 1998. Season of birth in psychiatry: a review. Neuropsychobiology 37:175–181.

10. (Castrogiovanni et al., 1998) Using other’s ideas or data is fine, but not citing where the information came from is plagiarism. Castrogiovanni P, Iapichino S, Pacchierotti C, Pieraccini F. 1998. Season of birth in psychiatry: a review. Neuropsychobiology 37:175–181.

11. SUBJECTS AND METHODS To investigate the influence of birth season on examination marks, we used the (anonymous) examination results from 1995–2001 of undergraduate students at the University of Vienna, together with their dates of birth……. Materials and methods or other synonymous sections detail how the experiments were done.

12. RESULTS The distribution of scores attained by male and female students is associated with their birth season (see data in Table 1)……… The results section details the outcomes of the experiments.

13. RESULTS The distribution of scores attained by male and female students is associated with their birth season (see data in Table 1)……… The results section details the outcomes of the experiments, and refers to the tables and figures in the paper.

14. Figures are graphical representations of data.

15. Sometimes tables are used.

16. DISCUSSION We find that examination scores are related to season of birth in both female and male students, indicating that there could be some biologically significant underlying ontogenetic or early life- history mechanism……… The discussion is where the results are explained and related to other research. (sometimes it is combined with the results)

17. What is in a journal article? Bonus #1 asks you to submit an article relevant to class.

18. How is information transferred between cells?

19. Fig 9.2 Different strains of bacteria are injected into mice. How is information transferred between cells?

20. Fig 9.2 How is information transferred between cells?

21. Fig 9.2 How is information transferred between cells?

22. Fig 9.2 How is information transferred between cells?

23. What has happened to the bacteria? Fig 9.2

24. DNA is the transforming agent Fig 9.3

25. If these two can win a Nobel prize… James Watson and Francis Crick The Structure of DNA

26. Rosalind Franklin Data showing uniformity of DNA structure. Fig 9.13

27. Fig 9.8 Nucleotides have a sugar backbone

28. Fig 9.8 This subtle difference in structure has profound effects.

29. Fig 9.8 Plus four different bases

30. Together with a phosphate = nucleotide Fig 9.9

31. Together with a phosphate = nucleotide Fig 9.9

32. Fig 9.11 Connect nucleotides by covalent bond = strand

33. Fig 9.17 DNA is typically double stranded The strands are connected by hydrogen bonds

34. Base pairing in DNA Figure 7-10 Fig 9.17

35. Two representations of the DNA double helix Figure 7-9 Fig 9.18

36. Fig 12.1 DNA stores information, but does not do anything. The information must be expressed to be useful.

37. a gene The relationship between DNA and genes promotercoding regionterminatornon-gene DNA

38. DNA Composition: In humans: Each cell contains ~6 billion base pairs of DNA. This DNA is ~2 meters long and 2 nm wide. ~97% does not directly code for amino acids In a single human cell only about 3-5% of genes are expressed at a time.

39. Width of DNA Length of human DNA in each cell DNA from a single human cell is ~2 meters long and 2 nm wide

40. DNA Composition: In humans: Each cell contains ~6 billion base pairs of DNA. This DNA is ~2 meters long and 2 nm wide. ~3% directly codes for amino acids ~10% is genes In a single human cell only about 5-10% of genes are expressed at a time.

41. a gene - DNA used to produce RNA or protein The relationship between DNA and genes promotercoding regionterminatornon-gene DNA

42. Five Perspectives of a Gene

43. Genes act as units of heredity…storing and passing on information.

44. Genes act as units of heredity… storing and passing on information.

45. Genes are seen as a cause of disease

47. Sickle-cell anemia is caused by a single nucleotide change in the hemoglobin gene Fig 16.1

48. Genes code for proteins Fig 12.1

49. Proteins are the “doers” of the cell. They act as: Enzymes Structural Support Transporters Signals Genes code for proteins…

50. Genes act as switches, controlling development

52. Genes are replicators (selfish gene) From “Biology 7th ed.” by Campbell et al fig 19.14

53. Fig 9.4 Viruses infect living cells, take over, and produce more virus.

54. Bodies are vessels for the transmission of genes

55. Five Perspectives of Genes: 1.Genes act as units of heredity 2.Genes are seen as a cause of disease 3.Genes code for proteins 4.Genes act as switches, controlling development 5.Genes are replicators (selfish gene)

56. Transposons

57. Genes are replicators (selfish gene) Transposons: mobile DNA Section 17.3

58. Barbara McClintock, discoverer of transposons

59. One type of transposon moves from place to place without increasing the number of transposons… Section 17.3

60. Fig 17.12C Retro-transposons replicate via an RNA intermediate, thereby increasing their number.

61. Transposons are self-moving DNA Fig 17.13

62. Transposons move within genomes via the action of transposase Fig 17.14

63. Fig 17.11 transposasetransposon

64. Fig 17.11

68. Genes are replicators (selfish gene) From “Biology 7th ed.” by Campbell et al fig 19.14

69. Genes are replicators (selfish gene) Transposons: mobile DNA Fig 17.13

70. Five Perceptions of Genes: 1.Genes act as units of heredity 2.Genes are seen as a cause of disease 3.Genes code for proteins 4.Genes act as switches, controlling development 5.Genes are replicators (selfish gene)

71. Protein DNA is used to produce RNA and/or proteins, but not all genes are expressed at the same time or in the same cells. How do cells control which genes are expressed?

72. What is in a scientific article plus DNA Structure and Function Bonus #1 is due 10/02/08. Bonus #2 is due 11/25/08.