1. RNA Processing

2. RNA, ribonucleic acid single strand polymers of ribonucleotides have secondary and tertiary structure

3. Classification of RNA Typesfunction ribosomal RNA (rRNA) messenger RNA (mRNA) transfer RNA (tRNA) heterogeneous nuclear RNA (hnRNA) small nuclear RNA(snRNA) small nucleolar RNA (snoRNA) small cytoplasmic RNA ribozyme microRNA (miRNA) small interfering RNA (siRNA) non-coding RNA (ncRNA) component of ribosome template of protein synthesis transfer amino acid premature mRNA participate the splicing and transfer of hnRNA, includes spliceosomal RNA process and modify rRNA recognize the protein sorting signal catalytic RNA usually endogenous, induce degradation of targeted mRNA usually exogenous, induce degradation of targeted mRNA all RNA other than mRNA, priorly functional RNA longer than 200nt

4. mRNA Processing of mRNA Structure of mRNA hnRNA m 7 GpppAAA ….A n 5’5’ 3’3’ AUGGUG …………………………… UAA 5 ’ -cap 5 ’ UTR coding region 3 ’ UTR 3 ’ poly(A) tail triplet code or codon

5. mRNA(cont.) The process of mRNA is on the base of hnRNP 5 ’ -cap and 3 ’ poly(A) play a role in the translocation of mRNA (from nuclear to cytoplasm), stability, control of protein translation. Life Cycle of an mRNA

11. RNA interference and siRNA