1. Animal Development Emily Huang, Erin McGrath, Michelle Xu

2. Review Cell differentiation Morphogenesis Blastula Gastrulation Cytoplasmic determinants

3. Fertilization Sperm + egg  combine to form diploid (zygote) – Contact of sperm to surface of egg initiates metabolic reactions within egg that trigger onset of embryonic development Acrosomal Reaction Cortical Reaction

5. Fertilization In Different Species Mammal -internal fertilization -eggs coated in follicle cells -fusion doesn’t not occur until after first cell division (12-36 hours) Echinoderm -external fertilization -eggs have a jelly coat -nuclei of sperm and egg fuse immediately -first cell division occurs 90 minutes after fusion Similarities -contact, acrosomal reaction (dig hole into egg), fusion of sperm and egg membranes, complete entry of sperm, cortical reaction blocks other sperm from coming in -sperm cell contains basal body which becomes the centrosome and allows zygote to split Differences

6. After Fertilization… …embryonic development proceeds through cleavage, gastrulation, and organogenesis. – Stage 1: cleavage  cell division creates a hollow ball of cells (blastula) from the zygote – Stage 2: gastrulation  produces 3 layered embryo (gastrula) – Stage 3: organogenesis  generates rudimentary organs from which adult structures grow

7. Cleavage Blastomeres: smaller cells that result from cleavage, each with its own nucleus First 5-7 divisions form a cluster of cells known as the morula Blastocoel: fluid filled cavity that begins to form within the morula Yolk: stored nutrients, most concentrated toward one pole, the vegetal pole and decreases toward the animal pole Afterwards, gastrulation occurs

8. Gastrulation Dramatic rearrangement! Forms primitive gut Driven by changes in cell motility, cell shape, and cellular adhesion to other cells and e.c. matrices 3 layers produced- collectively the gastrula Germ layers: ecto, meso, endo Sea Urchins:Frogs: Chicks:

9. Germ Layer Fun ECTODERM: -skin (sweat glands, hair follicles) -sensory receptors in epidermis -cornea, lens of eye -nervous system -tooth enamel ECTODERM: -skin (sweat glands, hair follicles) -sensory receptors in epidermis -cornea, lens of eye -nervous system -tooth enamel MESODERM: -skeletal system -muscular system -excretory system -circulatory and lymphatic systems -reproductive system -dermis of skin MESODERM: -skeletal system -muscular system -excretory system -circulatory and lymphatic systems -reproductive system -dermis of skin ENDODERM: -epithelial lining of digestive tract and respiratory system -lining of urethra, urinary bladder, repro. system -liver, pancreas, thymus, thyroid ENDODERM: -epithelial lining of digestive tract and respiratory system -lining of urethra, urinary bladder, repro. system -liver, pancreas, thymus, thyroid

10. Organogenesis Frog Chick When the three germ layers begin to develop into organs

11. Development Adaptations of Amniotes Reptiles, birds, and mammals are called amniotes. All vertebrate embryos require aqueous environment, so animals use fluid filled eggs or uteri

12. Extraembryonic Membrane Present in mammals, birds and reptiles. In birds and reptiles: germ layers develop into – Amnion- contains the fluid and fetus – Yolk sac, chorion, and allantois Allantois is a disposal sac for waste Yolk sac expands over yolk, stockpile of nutrients Exchange gases between embryo and surroundings These structures provide “life support system” Mammals have the same structures, only we have a uterus instead of an egg to contain them

13. US THEM (chicken)

14. Morphogenesis Cytoskeletons in animals cells allow them to “crawl” from place to place in all developing animals. Cell crawling changes the shape of the developing animal by extending or contracting- convergent extension

15. What affects developmental fate? Cytoplasmic determinants  cell differentiation Interactions among cells causes changes in gene expression  “induction”

16. Limb Formation and Spatial Organization Inductive signals play a major role in pattern formation Positional information: molecules that direct a cell to its correct location in the body In all animals with limbs: apical ectodermal ridge (AER), zone of polarizing activity (ZPA) They are major limb bud organizers

17. apical ectodermal ridge (AER)- a thick ridge of ectoderm at the tip of a bud of a limb, determines growth of the limb zone of polarizing activity (ZPA)- sprouts from the mesoderm, forms the digits ZPA excretes a protein growth factor called SONIC HEDGEHOG!!!!!!!!!!!!!!

18. THE END <3