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Published byEileen Greene Modified over 9 years ago
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GENETIC ENGINEERING The process of manipulating genes for practical purposes
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Recombinant DNA DNA created from 2 or more different organisms.
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Steps in a Genetic Engineering Experiment Cutting DNA- Restriction enzymes from bacteria are used to cut out portions of DNA Plasmids- circular DNA molecules that can replicate independently Recombinant DNA is made using ligase to piece the segments together Genes are cloned by the host Cells with the new DNA are seperated from those without the new DNA
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Confirmation of a Cloned Gene DNA from each clone is cut with restriction enzymes and sorted using gel electrophoresis.
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The Human Genome Project A research project designed to identify the encoding of human DNA Completed in 2003, the Human Genome Project (HGP) was a 13-year project coordinated by the U.S. Department of Energy and the National Institutes of Health. During the early years of the HGP, the Wellcome Trust (U.K.) became a major partner; additional contributions came from Japan, France, Germany, China, and others.
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Uses of Genetically Modified DNA Drugs- sometimes disorders and illness prevent the body from making critical proteins. Drug companies use this method of genetic engineering to make the proteins
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Other Uses Vaccines- solutions containing all or part of a harmless version of a pathogen. The immune system recognizes the surface proteins and responds by defensive proteins and antibodies.
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DNA Fingerprinting A pattern of dark bands on photographic film that shows the pattern of fragments separated by gel electrophoresis.
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Agricultural /Farm Uses More Nutritious Crops Larger meat producing animals Hardier plants that can survive climate changes -transgenic organisms Dolly the sheep
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Risks of GMO’s You Decide- Each table will read an article on genetic engineering. Collect information from the article, them make a poster about what you learned. You will share these in front of the class TODAY.
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