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Published byOwen Harper Modified over 9 years ago
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Genetic Engineering changing DNA within an organism
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This is what most people think of…. When they think about moving genes from one organism to another This IS NOT what happens!
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Genetic Technology Recombinant DNA (Takes DNA from one organism and places it into the DNA of another organism) This new organism is called a Trangenic Organism OR Genetically Modified Organism (GMO) an organism containing genes from a different organism
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6 Steps to Recombinant DNA 1.Remove Gene Remove the needed gene from the DNA Restriction enzymes are like scissors- used to cut out certain sections of DNA
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2.Prepare the Vector Vector: something that carries the DNA A plasmid is removed from a bacterial cell. (A plasmid is a circular piece of bacteria DNA)
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Restriction enzymes are used to cut the plasmid open. Leaving sticky ends OR pieces of single stranded DNA that stick off of the plasmid Draw one in your notes
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3.Insert the gene (DNA) into Plasmid using the sticky ends
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4.The recombinant DNA (plasmid and new gene) is inserted back into a bacteria cell.
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5.Bacteria Cells Reproduce When the bacterial cells reproduce by dividing, the inserted gene is also reproduced in the newly created cells.
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6.Bacteria Produce Proteins The protein that this new gene produces can be removed and used for a medicine, industrial, agricultural, or other uses.
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This is Real What if we take the gene that makes some jellyfish glow in the dark and add it to the DNA in the cells of another organism?
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