1. Wake-up 1.What do restriction enzymes do? 1.Restriction enzymes are naturally produced by bacteria. Explain why they have them. 2.Explain the difference between a blunt cut and a staggered cut.

2. Homework Check the answers on your homework. Find the link on the Wiki, “Genetic Engineering SG KEY”.

3. Applications of Genetic Engineering

4. Transgenic Organism An organism (animals, plants, and bacteria) that has been genetically altered.

5. Example #1: Diabetes and Insulin (Medical)

6. What is Insulin? Insulin (protein) helps glucose move from our bloodstream to our cells Glucose is from food that was consumed

7. What produces insulin? Pancreas Watch me!!!

8. Type 1 Diabetes: The Pancreas does not produce insulin

9. Diabetics need to take Insulin shots How does someone with Type 1 Diabetes get insulin?

10. History of Insulin Production 1974 – Pancreas was removed from slaughtered animals and purified for human use

11. History of Insulin Production (finish) 1980’s – Gene that makes insulin in humans was inserted into E.coli (Bacteria) which produced synthetic human insulin

12. Example #3: Genetically Modified Food (GMO’s)

13. What are Genetically Modified Organisms (GMO’s)? An organism whose genetic material has been altered using genetic engineering techniques

14. Why are Organisms Genetically Modified? Modified to be resistant to: Pests Disease Cold Drought Salt Also an overall “better” quality

15. Example #1 of GMO: Golden Rice

16. Malnutrition is common in 3 rd world countries; impoverished peoples rely on rice for the main staple of their diet.

17. However, rice does not contain adequate amounts of all necessary nutrients to prevent malnutrition More than 10 million children die of malnutrition every year. A vast majority of these deaths is linked to micronutrient deficiencies.

18. Vitamin A deficiencies can damage the immune system and decrease the body’s ability to resist or fight infections or can lead to partial to total blindness

19. Golden rice was genetically engineered to contain beta carotene, which can provide Vitamin A (Video: Beginning to 4:30) Watch me!!!

20. GMO Example #2: Tomato and Artic Flounder Video Clip Watch me!!!

25. Example #3: Glowing Organisms (purpose unclear)

26. Which organisms naturally glow?

29. Can we make other organisms glow using Genetic Engineering?

42. Genetic Modified Organisms Watch me!!!

43. When complete with notes, pick up the Paper Plasmid activity from the substitute. Also grab scissors and a few pieces of tape. (Leave tape dispensers where they are, just take tape) Work through the activity. Make sure to read the directions carefully. When complete, answer the questions on the lab. Before you turn it in, staple the Recombinant DNA with the insulin gene to your lab sheet. Pick up your homework and work on it.