1. M201
Microplate Reader
This power point file is prepared mainly for the engineers of our distributors.

2. Advice for this course
You needn’t make a detailed note, especially with the diagrams.
You can copy and keep this file, then review it anytime.
Pay more attention to my explain can help you understand it clearly.
Some diagrams are truly hard to draw by hand ^_^.
There will be a short pause after each section.
You can ask some questions about that section then, I would do my best to answer you.
Please remember the of the product specialist: if you have some further questions, suggestions, please send an .

3. Basic principle
Antigen will combine only with the specific antibody

4. ELISA test Antibody Plate Well Antigen (from sample) Enzymes Enzymes
Substrate
Colored product
catalyzing

5. Steps combine catalyzing combine wash wash Stop buffer wash Read
Antibody
wash
Sample
wash
catalyzing
Stop buffer
Substrate
wash
Antibody-Enzyms
combine
Read

6. Advantages Mono-LED light source Based on Windows CE.net
7” TFT colorful LCD screen. Optional touch screen supported.
Membrane keyboard with numeric keys and short-cut function keys.
Multi-test (different tests on one plate)
Test, calibrate, QC on one board
Fast reading (less than 5s for whole plate)
Large memory:1200 plates (96 wells).

7. (bottom side) LED board
Inner view
Keyboard
7” LCD display
Printer board
Main fan
Core board
Back end board
Power supply
仪器的构成
上盖上面的部件主要为显示器，键盘和打印机，都是常见的输入输出设备，在此不做介绍
拆掉上盖后就能看到这张图片所示的内部结构：
主要有 电源盒，主风扇，前端板，后端板，核心板，转接板，泵，光源模块，比色模块，滤光模块等
Transfer board
Plate tray
(bottom side) LED board
motor & detectors
Front end board

8. Functional Block Diagram 1
LED Array 8x4 (+2)
Keyboard
Light sensor x8
LED
motor
Plate motor
Front-end
Union
LED steps
detector
Plate steps detector
LED origin
detector
Plate origin
detector
Control signals
from back end

9. Functional Block Diagram 2
Data from front end
light intensity and
detector signal, etc.
Touch
screen PC
External Keyboard
LCD and buzzer
Back-end
union
Mouse
Thermal
printer
Barcode
reader
Standard PCL printer
USB
disk SD
card

10. LED Array 405 450 492 630 Optional x2 8 Channels
Patent: mono-LED illumination.
More than 100,000 hours long life.
Needn’t replace lamp any more.
No stabilizing time.
Device can be fast turned on.
Twinkling reading.
Low power and low heat.

11. Wavelength control Front-end board LED Array Opto-coupler
珀尔贴元件示意图
我们可以通过切换电压方向来让升温或降温。
Opto-coupler
Origin detector
Opto-coupler
Step detector

12. Microplate EMP-168兼容比色池和比色杯两种液体模式 比色池模式有以下优势 操作简单，操作时不需要频繁更换；
寿命长，比色池和杯座都能使用较长时间
经济，使用少量的试剂和样本就能进行测量

13. Plate position control
Opto-coupler
Step detector
Front-end board
Hall element
Origin detector

14. Breakdown Description
Troubleshooting 1
Breakdown Description
Solutions
Device can not start
check the power plug
check the fuse
check the power supply
Cannot pass Self-check
make sure the plate carrier is empty
restart the machine
check the optical path
Results abnormal
1. check if the plate lay correctly
. check the test parameters
detect the standard material
Plate cannot move
check if the plate is in position
check if there is something on the block
check if the plate carrier can move manually
The results of individual channel abnormal
check weather the result is normal when not put micro perforated panel
check if there are some lens loose
check if any LED damaged
Mouse cannot move
reconnect the mouse
test the mouse on PC
常见故障解决办法，这里的内容在我们的服务手册上可以找到。
部分故障的具体解决办法，参考我们编制的指导文件。

15. Breakdown Description
Troubleshooting 2
Breakdown Description
Solutions
Thermal printer cannot work
Make sure the internal printer be selected in system setting
Check if the printer paper is installed
External printer cannot work
make sure the internal printer be selected in system setting
check if the USB cable connected well
check if the printer type can be supported by machine
Screen is blank with back light on
check if the power equipment started property
check the power supply
check the FFC cable
Keyboard cannot work
check it he keyboard cable connection
check if the voltage in frond-end normal
Motor make noise
check the plate carrier
check weather the detection opocouple board loose
check if there is some foreign matter

16. Please read user’s manual and service manual for more information.
Thank you!
Copyright © Shenzhen Emperor Electronic Technology Co., Ltd.
1st edition, June, 2012