1. Summer Research 2012 Studying the metabolic pathway of Acidobacterium Capsulatum Sunny Ketchum, Pariss Trujillo, and Professor Les Sommerville

3. Figure 1.- A. Capsulatum growth and glucose concentration It was found that A.Cap grew on one sole carbon source, glucose, at an exponential rate until all glucose was consumed.

4. Figure 2.- Turbidity, pH, and protein concentration as a function of time in hours A.Cap reached its peak growth after 75 hours. This displays the amount of protein found in A.Cap along with the growth environment after a certain amount of hours.

5. * Thauer RK, Jungerman K, Decker K. Energy Conservation in Chemotrophic Anaerobic Bacteria. Bacteriological Reviews (41)1, 1977, 100-180. Biomass Estimate From Protein Concentration 600 mg dry weight (assuming 15% protein and 1% inorganic ions by wet cell weight) 1500 mg – 600 mg – 600 mg = 300 mg POLYSACCHARIDE EXCRETED.9 g x (1 mol ATP/9 g cells) x (1 mole glucose/30 moles ATP) x (180 g glucose/mole) x (1000 mg/1 g) = 600 mg GLUCOSE USED FOR ENERGY * (including energy needed for polysaccharide synthesis) 1500 mg glucose in media at zero hours

6. Quenching and Extraction of Water Soluble Metabolites A series of NaCl and 35% per chloric acid rinses were used on the cells in order to quench and extract the water soluble metabolites. This gave a clearer H’NMR spectra. The H’NMR peaks were then identified for metabolites.

7. H’NMR spectra of A.Cap in exponential phase

8. H’NMR spectra of A.Cap in stationary phase