1. Safe Clinical Practice

2. Keys to Lab Safety and Success Be Prepared

3. Keys to Lab Safety and Success Equipment

4. Keys to Lab Safety and Success Attendance

5. Keys to Lab Safety and Success Writing

6. Keys to Lab Safety and Success Curiosity

7. Lab Skills for Success Communication

8. Lab Skills for Success Teamwork

9. Lab Skills for Success Critical Thinking

10. American Philosophical Association Critical thinking is self-directed, self- disciplined, self-monitored, and self- corrective thinking. It presupposes assent to rigorous standards of excellence and mindful command of their use. It entails effective communication and problem solving abilities and a commitment to overcome our native egocentrism and sociocentrism.

11. Lab Skills for Success Technical skills

12. Infection Prevention & Control Micro-organism – Only visible with a microscope

13. Micro-organism Cause disease “pathogen”  infection Do not cause disease “Nonpathogenic organism” Normal Flora

14. Cross-contamination Spread of pathogens from  

15. Bacteria Single cell No nucleus Reproduce Classifications – Need for O 2 – Shape – Gram stain

16. Bacteria – need for O2 Aerobic Need O2 Anaerobic Can’t have O 2

17. Bacteria – gram stain Gram-positiveGram-negative Dangerous  Endotoxin  Hemorrhagic shock & diarrhea

18. Bacteria – shape (morphology) Cocci  – Round Bacilli  – Rod shaped Spirochetes  – Spiral Chains  – Streptococci Pairs  – Diplococci Clusters  – Staphylococci

19. Antibiotic Chemical substances that can kill microorganisms Most effective against bacteria

20. Viruses Extremely small Can only grow and replicate within a living cell

21. Chain of Infection 1.Pathogen Microorganism capable of causing disease

22. Chain of Infection 1.Pathogen 2.Reservoir 3.A portal of exit Reservoir – Where the pathogen lives Portal of Exit – A way to get out of the reservoir

23. Chain of Infection 1.Pathogen 2.Reservoir 3.Portal of exit from the reservoir 4.Mode of Transmission How pathogens move from the reservoir  host

24. Chain of Infection 1.Pathogen 2.Reservoir 3.Portal of exit from the reservoir 4.Mode of Transmission 5.Portal of entry Entrance into host – Eyes – Mouth – Respiratory – GI – GU – Broken skin – Blood

25. Chain of Infection 1.Pathogen 2.Reservoir 3.Portal of exit from the reservoir 4.Mode of Transmission 5.Portal of entry Susceptible host

26. Healthcare-associated infections Mode of Transportation – Employees – Visitors – Devices

27. Resistant pathogens Causes – Inappropriate use of antibiotics Result – Resistance – Mutation

28. Methicillin-Resistant Staphylococcus Aureus (MRSA) Reservoir – Mucous membranes – Resp. Tract Hospital & community acquired Isolation – Precautions

29. Standard Precaution Do not touch – Blood – Body fluids (except sweat) – Non-intact skin – Mucous membranes

30. Hand washing Before & After – Soap

31. Gloves Before touching – Body fluids

32. Gown During procedures when contact with body fluids is anticipated.

33. Mask – Eye protection – Face shield When body fluids are likely to splash or spray.

34. Equipment Wash hands after handling Cleaning routine

35. Laundry/textiles Cover Don’t hug

36. Needles / sharps Do not recap 1-handed scoop Puncture- resistant container

37. Lifting & moving Obtain help

38. Lifting & moving Use your leg muscles

39. Lifting & moving Provide stability for movement

40. Lifting & moving Smooth, coordinated movements

41. Lifting & moving Keep the load close

42. Lifting & moving Pull & pivot