2. 600 400 200 DNA Function DNA Tech 600 400 200 Potpourri FINAL JEOPARDY! FINAL JEOPARDY!

3. DNA Function for 200 During DNA replication, a template strand reads TTTAGCTAGCTA. What is the sequence of the new strand? AAATCGATCGAT

4. DNA Function for 400 What sequence of mRNA makes up the start codon? What is the anticodon of the corresponding tRNA? AUG UAC

5. DNA Function for 600 When translation is complete, what might immediately happen to the… mRNA = translated again by another ribosome to make additional copies of the resulting protein Polypeptide = will be shaped into a functional protein tRNA = will pick up another matching amino acid and bring it to another ribosome

6. DNA Tech for 200 What is the specific original purpose of restriction enzymes? They are produced by bacteria to destroy the DNA of invading viruses

7. DNA Tech for 400 What is the major functional difference between adult stem cells and embryonic stem cells? ASCs are pluripotent. They can divide to produce a few different types of somatic cells. ESCs are totipotent. They can divide to produce any cell in the body (or a whole new embryo).

8. DNA Tech for 600 What is the purpose of heating a sample in the process of PCR? It denatures the DNA which means that the hydrogen bonds holding together complementary bases of opposite strands break. This separates the strands.

9. Potpourri for 200 How many unique nucleotide variations exist in our cells? 8 4 DNA nucleotides with A, C, G, or T 4 RNA nucleotides with A, C, G, or U

10. What is transformation, and why is it an important step in gene cloning? It is the absorption of foreign plasmid DNA into bacterial cells. Once the plasmid is absorbed, the bacteria can express the new genes, and they copy the whole plasmid whenever they carry out binary fission. Potpourri for 400

11. Potpourri for 600 How is it that a mutation might occur in the DNA, but there is no corresponding change in the organism’s phenotype? 1) The mutation may not be within a gene. 2) The mutation may be transcribed into a different mRNA codon, but it may still code for the same amino acid.

12. Draw the gel from the data SampleChargeSize 1+2000 2-3000 3+1000 4-4000 + - 1 2 3 4