1. Presentation Title April 4, 2002 CAMERA- Metagenomics meets the Cyberinfrastructure David T. Kingsbury Gordon and Betty Moore Foundation BERAC - October 16, 2006

2. Presentation Title April 4, 2002 The CAMERA Partnership Community Cyberinfrastructure for Advanced Marine Microbial Ecology Research and Analysis

3. Presentation Title April 4, 2002 Genomic Data Is Growing Rapidly, But Metagenomics Will Vastly Increase The Scale… GenBank Protein Data Bank www.rcsb.org/pdb/holdings.html www.ncbi.nlm.nih.gov/Genbank 100 Billion Bases! Total Data < 1TB 35,000 Structures

4. Presentation Title April 4, 2002 The Sargasso Sea Experiment The Power of Environmental Metagenomics  Yielded a Total of Over 1 billion Base Pairs of Non-Redundant Sequence  Displayed the Gene Content, Diversity, & Relative Abundance of the Organisms  Sequences from at Least 1800 Genomic Species, including 148 Previously Unknown  Identified over 1.2 Million Unknown Genes MODIS-Aqua satellite image of ocean chlorophyll in the Sargasso Sea grid about the BATS site from 22 February 2003 J. Craig Venter, et al. Science 2 April 2004: Vol. 304. pp. 66 - 74

5. Presentation Title April 4, 2002 Marine Genome Sequencing Project Measuring the Genetic Diversity of Ocean Microbes

6. Presentation Title April 4, 2002 Moore Foundation Funded the Venter Institute to Provide the Full Genome Sequence of 155 Marine Microbes www.moore.org/microgenome/trees_main.asp

7. Presentation Title April 4, 2002 Moore Microbial Genome Sequencing Project: Cyanobacteria Being Sequenced by Venter Institute

8. Presentation Title April 4, 2002 Moore Microbial Genome Sequencing Project: Cyanobacteria Being Sequenced by Venter Institute

9. Presentation Title April 4, 2002 GOS Sequences are Largely Bacterial Source: Shibu Yooseph, et al. (PLOS Biology in press 2006) ~3 Million Previously Known Sequences ~5.6 Million GOS Sequences

10. Presentation Title April 4, 2002 Metagenomics Will Couple to Earth Observations Which Add Several TBs/Day Source: Glenn Iona, EOSDIS Element Evolution Technical Working Group January 6-7, 2005

11. Presentation Title April 4, 2002 Driven by User Needs  CAMERA serves as one representation of a specific research community’s need for a system to –Collect and reference increasing metadata relevant to environmental metagenome datasets –Exploit the power of querying on metadata across multiple geospatial locations –Have access to a diverse and customizable set of easy-to-use tools to analyze their data in the context of collected metagenomic and whole genomic datasets –Have the ability to update and propagate improvements to annotations –Have a pre-publication, pre-submission collaborative workspace –Serve a diverse informatics-literate community

12. Presentation Title April 4, 2002 Services Provided  Data and Application Services  Tools and Workflows  Computational Data, Visualization and Collaborative environment  Outreach and Training in Environmental Genomics

13. Presentation Title April 4, 2002 Data and Application Services  Primary Data –Sargasso Sea and Sorcerer II expedition data –JGI marine & terrestrial environmental datasets –Moore Microbial Genomes –JGI and other relevant whole genomes –Research community submitted datasets –Submitted 454-based metagenomic datasets –Publically available NR protein and DNA sequence datasets  Derived Data –Annotations of datasets –Assemblies –Alignments –Pre-computed clusters

14. Presentation Title April 4, 2002 Sample Metadata from GOS  Site Metadata –Location (lat/long, water depth) –Site characterization (finite list of types plus “other”) –Site description (free text) –Country  Sampling Metadata –Sample collection date/time –Sampling depth –Conditions at time of sampling (e.g., stormy, surface temperature) –Sample physical/chemical measurements (T (oC), S (ppt), chl a (mg m-3), etc) –“author”  Experimental Parameters –Filter size –Insert size

15. Presentation Title April 4, 2002 Tools and Workflows  Initial set –BLAST Server –Clustering –HMM/Profile –Neighborhood analysis –Multiple sequence alignments –Assembly  Proposed New Tools –Multiple Auto Annotation pipelines –Fast Sequence lookup –Customized Assembly –Phylogenetic Analysis –Clustering Tools

16. Presentation Title April 4, 2002 CAMERA Outreach Modes  Scientific Advisory Board –Early Adopters – OptIPortal End Points  Targeted Workshops –User Forums –User Software Testing –Viz Tool Brainstorming  Presentations at Scientific Meetings –e.g. Demonstration Booth at JCVI Genomes, Medicine, and the Environment Conference October 2006  Partnerships With Metagenomics Projects –E.g. DoE’s Joint Genome Institute (JGI)  Training and User Services Team

17. Presentation Title April 4, 2002 Guiding Philosophy for Development  Sprint Q4 2006 –Propagate JCVI toolkit and data ASAP  Mechanism for publication of Sorcerer II data  Enabler for community –Defined deliverables, project management approach  MarathonQ4 2006 onward –Additional Datasets –Additional tools –Community drives prioritization for ongoing releases  Advisory Board, Community Outreach  Keys to success:  Tight integration of science, bioinformatics, software, and IT  Matched to Community Needs

18. Presentation Title April 4, 2002 The Future Home of the Moore Foundation Funded Marine Microbial Ecology Metagenomics Complex First Implementation of the CAMERA Complex Photo Courtesy Joe Keefe, Calit2 Major Buildout of Calit2 Server Room Underway http://calit2-1101-1.ucsd.edu/

19. Presentation Title April 4, 2002 Moore CAMERA Production Environment  Creation of Initial Production Environment – September 2006 –Hardware  Compute Nodes – –~200 4 CPU Nodes = ~800 Processing Cores  Storage Servers – –10 systems = ¼ Petabyte raw storage  Database Servers –Larger 20-40TB; Smaller 5-10TB  Network Management – –Force10 E1200 Router w/12 10GigE Interfaces to Each System Ports  User Access to Compute Cycles –Bulk of free cycles available to external users –Proposal mechanism Source: Greg Hidley, Calit2; Phil Papadopoulos, SDSC, Calit2

20. Presentation Title April 4, 2002 www.glif.is Created in Reykjavik, Iceland 2003 Countries are Aggressively Creating Gigabit Services: Interactive Access to CAMERA and LOOKING Systems Visualization courtesy of Bob Patterson, NCSA.

21. Presentation Title April 4, 2002 Scale

22. Presentation Title April 4, 2002 Flat File Server Farm W E B PORTAL Traditional User Response Request Dedicated Compute Farm (1000 CPUs) TeraGrid: Cyberinfrastructure Backplane (scheduled activities, e.g. all by all comparison) (10000s of CPUs) Web (other service) Local Cluster Local Environment Direct Access Lambda Cnxns Data- Base Farm 10 GigE Fabric Calit2’s Direct Access Core Architecture Will Create Next Generation Metagenomics Server Source: Phil Papadopoulos, SDSC, Calit2 + Web Services Sargasso Sea Data Sorcerer II Expedition (GOS) JGI Community Sequencing Project Moore Marine Microbial Project NASA Goddard Satellite Data Community Microbial Metagenomics Data

23. Presentation Title April 4, 2002 OptIPuter Scalable Adaptive Graphics Environment (SAGE) Allows Integration of HD Streams OptIPortal– Termination Device for the OptIPuter Global Backplane

24. Presentation Title April 4, 2002 OptIPortal– Termination Device for the OptIPuter Global Backplane  20 Dual CPU Nodes, 20 24” Monitors, ~$50,000  1/4 Teraflop, 5 Terabyte Storage, 45 Mega Pixels--Nice PC!  Scalable Adaptive Graphics Environment ( SAGE) Jason Leigh, EVL-UIC Source: Phil Papadopoulos SDSC, Calit2

25. Presentation Title April 4, 2002 UIC/UCSD 10GE CAVEWave on the National LambdaRail Emerging OptIPortal Sites CAVEWave Connects Chicago to Seattle to San Diego…and Washington D.C. as of 4/1/06 and JCVI as of 5/15/06 NEW! SunLight CICESE UW JCVI MIT SIO UCSD SDSU UIC EVL UCI OptIPortals

26. Presentation Title April 4, 2002 First Remote Interactive High Definition Video Exploration of Deep Sea Vents Source John Delaney & Deborah Kelley, UWash Canadian-U.S. Collaboration

27. Presentation Title April 4, 2002 High Definition Still Frame of Hydrothermal Vent Ecology 2.3 Km Deep White Filamentous Bacteria on 'Pill Bug' Outer Carapace 1 cm. Source: John Delaney and Research Channel, U Washington

28. Presentation Title April 4, 2002 A Near Future Metagenomics Fiber Optic-Enabled Data Generator Source John Delaney, UWash