2. Metabolism Metabolism involves two main processes, catabolism and anabolism Catabolic reactions break down large, complex molecules to provide smaller molecules and energy (ATP) Anabolic reactions use ATP energy to build larger molecules from smaller building blocks

3. Stages of Catabolism Catabolic reactions are organized into three stages: In Stage 1, digestion breaks down large molecules into smaller ones that enter the bloodstream In Stage 2, molecules enter the cells and are broken down into two- and three-carbon compounds In Stage 3, compounds are oxidized in the citric acid cycle to provide energy (ATP) for anabolic processes

4. Stages of Catabolism (Diagram)

5. Eukaryotic Cell Structure Metabolic reactions occur at specific sites within the cell

6. Components of Eukaryotic Cells

7. ATP and Energy In cells, energy is stored in adenosine triphosphate (ATP) - there are other cellular energy sources, but ATP is the main one

8. Hydrolysis of ATP The hydrolysis of ATP to ADP releases 7.3 kcal (31 kJ/mole) ATP  ADP + P i + 7.3 kcal (31 kJ/mole) The hydrolysis of ADP to AMP releases 7.3 kcal (31 kJ/mole) ADP  AMP + P i + 7.3 kcal (31 kJ/mole)

9. ATP and Muscle Contraction Muscle fibers contains filaments of actin and myosin When a nerve impulse increases [Ca 2 +], the filaments slide closer together to contract the muscle The hydrolysis of ATP in muscle provides the energy for contraction As Ca 2+ and ATP decrease, the filaments return to the relaxed position

10. Coenzyme NAD + When a compound is oxidized by an enzyme, 2H as 2H + and 2e - are removed by a coenzyme, which is reduced NAD + (nicotinamide adenine dinucleotide) participates in reactions that produce a carbon-oxygen double bond (C=O) For example, NAD + participates in the oxidation of ethanol:

11. Structure of Coenzyme NAD + NAD + (nicotinamide adenine dinucleotide) contains ADP, ribose, and nicotinamide (from niacin, B 3 ) NAD + reduces to NADH when the nicotinamide group accepts H + and 2e -

12. Coenzyme FAD FAD participates in reactions that produce a carbon-carbon double bond (C=C) Oxidation —CH 2 —CH 2 —  —CH=CH— + 2H + + 2e - Reduction FAD + 2H + + 2e -  FADH 2

13. Structure of Coenzyme FAD FAD (flavin adenine dinucleotide) contains ADP and riboflavin (vitamin B 2 ) FAD reduces to FADH 2 when flavin accepts 2H + and 2e -

14. Coenzyme A (CoA) CoA activates acyl groups, such as the two-carbon acetyl group for transfer to other compounds It consists of pantothenic acid (vitamin B 5 ), phosphorylated ADP and an aminoethanethiol

15. Digestion of Carbohydrates (Stage 1) In the mouth, salivary amylase hydrolyzes  -glycosidic bonds in polysaccharides to give smaller polysaccharides (dextrins), maltose, and some glucose In the small intestine, pancreatic amylase hydrolyzes dextrins to maltose and glucose The disaccharides maltose, lactose, and sucrose are hydrolyzed to monosaccharides in the small intestine The monosaccharides enter the bloodstream - fructose and galactose are transported to the liver, where they are isomerized to glucose - glucose is transported to cells for metabolism

16. Overview of Stage 1 of Carbohydrate Catabolism

17. Glycolysis (Stage 2) In Stage 2 of carbohydrate catabolism, the metabolic pathway called glycolysis degrades glucose (6C) obtained from digestion to pyruvate (3C) Glycolysis is an anaerobic process that takes place in the cytoplasm

18. Energy-Investing Phase of Glycolysis In reactions 1-5 of glycolysis: Energy is used to add phosphate groups to glucose and fructose Glucose is converted to two three-carbon molecules

19. Energy-Producing Phase of Glycolysis In reactions 6-10, the hydrolysis of phosphates generates four ATP molecules Two NAD + coenzymes are also reduced

20. Glycolysis, Overall Reaction Glycolysis generates 2 ATP and 2 NADH Two ATP are used in energy-investment to add phosphate groups to glucose and fructose-6-phosphate Four ATP are formed in energy-generation by direct transfers of phosphate groups to four ADP Overall Reaction: Glucose + 2ADP + 2P i + 2NAD +  2Pyruvate + 2ATP + 2NADH + 4H +

21. Regulation of Glycolysis The amount of glucose that goes through glycolysis is regulated based on relative levels of ATP, ADP and AMP, as well as other glycolysis intermediates This regulation takes place at three steps: - Reaction 1: Hexokinase is inhibited by high levels of glucose-6-phosphate, which prevents the phosphorylation of glucose - Reaction 3: Phosphofructokinase, an allosteric enzyme, is inhibited by high levels of ATP and activated by high levels of ADP and AMP - Reaction 10: Pyruvate kinase, another allosteric enzyme is inhibited by high levels of ATP or acetyl CoA