1. Enzymes A review of how they work

2. Generate a definition of Enzymes Proteins that promote specific reactions in cells by lowering activation energy required to start a reaction. http://highered.mcgraw- hill.com/sites/0072495855/student_view0/chapter2/animation__ how_enzymes_work.html

3. How do enzymes work? Lower activation energy increase speed of metabolic rates Break down….build up???

4. Anatomy of Enzyme Action

5. Induced fit model Enzymes are specific http://www.phschool.com/science/biology_place/labben ch/lab2/induced.htmlhttp://www.phschool.com/science/biology_place/labben ch/lab2/induced.html

6. What happens after an enzyme does its job? Required in small amounts because as they work, not consumed.

7. Factors that affect how enzymes work? Temperature pH Amount of substrate available

8. Brainstorm of Enzymes Saliva Stomach Nervous system: AcHase Milk products Papaya fruit Unprocessed fruits Kiwi: actiniclin Soybeans (lipoxygenase) Fireflies (luciferase) Pineapples (bromelain) Potatoes Egg whites Turnips (peroxidase) Tears Yeast (sucrase) Lactase (sold overcounter) Onions Detergents Catalase: liver

9. How do you know “enzymes” are working? http://www.enetez.com/images/default/for_sale.jpg

10. Based on the analogy… 1.What is the product? –pizza 2.What are the enzymes? –workers 3.What is the active site of the enzymes? –Hands of pizza makers 4.What is the substrate? –toppings

11. Challenge Design an experiment that will test the effectiveness of an enzyme. Stipulations –Experiment will be performed in class period –conduction is totally designed by experimenters –Enzyme must be available to science lab –Data collection must be qualitative and quantitative Review scoring guide for how you will be graded