2. You are asked to attend assessment of a newborn of a 33-week gestation whose estimated birth weight is 1800 g. The mother is a 26-year-old G5,P4+0 who was admitted in active labour 2hours ago. The mother did not seek any prenatal care during pregnancy.

3. She reports no medical problems during the pregnancy. She denies tobacco, alcohol and illicit drug use. Mother's blood type is O+, antibody negative. Membranes are ruptured at the time of delivery revealing clear amniotic fluid.

4. At delivery, the baby is small male who was brought to the warming table. He is pink with good respiratory effort and his heart rate is 150 beats per minute. But, You note his skin is mildly jaundiced with raised red/purple lesions. So, you inform the mother that because you suspect the infant has a congenitally acquired infection, you are transferring him to the special care nursery for a more detailed examination and further management.

5. P/E of Newborn: Vital signs: Temperature: 37.5◦C. Pulse: 120 bpm. RR: 40 breath/min. BP: 60/36 mmHg. O2 saturation: 100% in room air. Growth parameters: Weight: 1854 g. Length: 44 cm. Head circumference: 31.5 cm.

6. P/E of Newborn: He is a small, thin male infant. Has little subcutaneous fat. He is in no acute distress. Skin is mildly jaundiced with the "blueberry muffin" appearance of diffuse raised red/purple lesions and petechiae. His anterior fontanelle is soft, but full. Abdominal Exam: Distended. A firm liver edge is felt 4 cm below the right costal margin and the spleen is felt 3 cm below the left costal margin.

7. Lab.: CBC: Moderate anemia. Thrombocytopenia. Lymphocytosis. LFTs: Liver enzymes are elevated. Direct bilirubin: 8mg/100ml “Elevated”.

8. Three hours after birth, the infant develops generalized tonic-clonic seizures that stop after administration of 20mg/kg of phenobarbital. Cranial CT: Periventricular calcification.

9. You suspected congenital infection with: CMV So, you consulted ophthalmologist to evaluate the patient for chorioretinitis.

10. Infections acquired in utero or during the birth process. They are a significant cause of fetal and neonatal mortality. Also, they are an important contributor to congenital malformation.

11. The concept of the perinatal infections is to group five infections in an acronym “TORCH”. T: Toxoplasmosis. O: Other; e.g., syphilis, parvovirus B19,… R: Rubella. C: CMV. H: Herpes simplex virus HSV. These infections have similar presentations, including rash and ocular findings.

12. When do you think of TORCH infections? IUGR infants. HSM. Thrombocytopenia. Unusual rash. Concerning maternal history. “Classic” findings of any specific infection.

13. Good maternal/prenatal history: Remember most infections of concern are mild illnesses often unrecognized. Thorough exam of infant. Directed labs/studies based on most likely diagnosis.

15. Toxoplasma gondii is a protozoan Organism exists in three forms Trophozoite Cyst Oocyst The incidance of congenetal toxplasma is0.3 to 1 /1000 live birth

18. Most infections are asymptomatic((70%)) When symptoms are present, they are will be ocular(76%) more than CNS manifestaion(52%) The ocular manifestation is chorioretinitis,optic atrophy, microphthalmia>>>>>blindness((use fundoscope))

19. CNS manefitation is hydrocephlus, intellctual and mental retardition, SNHL, seizure The triad of toxoplasmosis is Hydrocephalus Chorioretinitis Intracranial calcification>>do CT scan

20. Do cordocentisi and plcental sample Serology Toxoplasma IgG antibody IgM fluoresent antibody

21. Treatment of mother while fetus is still in utero Early treatment of the infant Compination of sulfadizin, pyramethamin, folic acid In pregnancy give her spyramycin

22. Use precautions when handling cat litter box Do not eat inadequately cooked meat

23. Congenital toxoplasmosis – key is avoidance of exposure during pregnancy

26. caused by an RNA Togavirus. ssRNA virus. Vaccine-preventable disease. Transmission: To mother: respiratory droplets. To fetus: Transplacental. The greatest risk for transmission to the fetus is during the 1 st trimester.

27. . Neonatal Manifestations IUGR low birth weight - prematurity stillbirth - spontaneous abortion Early Manifestations cloudy corneas Cataracts microcephaly hepatomegaly splenomegaly jaundice pulmonary valve stenosis patent ductus arteriosus thrombocytopenia purpura

29. Sensorineural Hearing Loss PDA Cataract

30. Effect on the Mother: Rash. Fever. Lymphadenopathy. Arthralgia.

31. Diagnosis : Virology Can isolate virus from nasal secretions Less frequently from throat, blood, urine, CSF Serology fetal rubella-specific IgM persistence of rubella-specific IgG after 8-12 months of age

32. No specific treatment. Avoid rubella vaccine during pregnancy

36. Defined as the isolation of CMV from the saliva or urine within 3 weeks of birth. member of the herpesvirus [ DNA ] Most common congenital viral infection Leading cause of sensorineural deafness Major cause of mental retardation, cerebral palsy CMV doesnot affect organogenesis but affects organ already developed Approximately 0.15–2% of live births

37. - Fetus: Via placenta from the mother - Human milk - Blood transfusion, organ transplantation - Children and adults: Mainly via bodily fluids (esp. urine, saliva) - For pregnant women, the two most common exposures to CMV are through sexual contact and through contact with the urine and saliva of young children with CMV infection. How is CMV transmitted?

38. CNS Manifestation : 70% microcephaly 60% intellctual impairment 35% sensorineural hearing loss 7% sizure

39. Systemic manifestation: Hepatosplenomegaly (70%) Jaundice (68%) (65%) Thrombocytopenia with Petechiae Chorioretinitis (20%)

40. SEQUELAE OF SYMPTOMATIC CONGENITAL CMV INFECTION Seizures Chorioretinitis Periventricular calcifications Sensorineural hearing loss motor deficits

41. CHORIORETINITIS

42. Congenital CMV

43. CHARACTERISTICS ASSOCIATED WITH INCREASED RISK OF SEQUELAE Primary maternal infection Symptomatic congenital CMV infection Presence of neonatal neurological abnormalities Abnormal head CT scan Chorioretinitis in the newborn

44. Isolation of CMV from urine or other body fluid (,CSF, blood, saliva) in the first 21 days of life. [gold standard] Serologic tests; CMV-specific IgM. (false +ve, false –ve ) PCR

45. Evaluation of mothers at risk of transmitting CMV to the fetus Refer for prenatal diagnosis

46. If your baby is diagnosed with congenital CMV infection, you should have his or her hearing and vision checked regularly. There is some evidence that ganciclovir, an antiviral drug, may prevent hearing loss and developmental outcomes in infants born with symptomatic congenital CMV infection with central nervous system involvement.

47. Here are a few simple steps you can take to avoid exposure to saliva and urine that might contain CMV : Wash your hands often with soap and water for 15-20 seconds, especially after changing diapers feeding a young child wiping a young child’s nose or drool handling children’s toys Do not share food, drinks, or eating utensils used by young children Do not put a child’s pacifier in your mouth Do not share a toothbrush with a young child Avoid contact with saliva when kissing a child Clean toys, countertops, and other surfaces that come into contact with children’s urine or saliva