2. 1990 Heart transplant 1990 Terminally-ill journalist Adrian Sudbury is campaigning to educate young adults about what it means to be a bone marrow donor

3. Henry is interested in working with orthopedics, which includes limb reattachment. Marci wanted to understand why educated adults are not more interested in being organ donors.

4.  Blood type  Genetic markers  What kind of organ/tissue  Immunoglobulins IgG,IgM,IgA  Age and sex of the giver and receiver of certain organs. Immunosuppressant drugs can change everything!

5.  IgG:  2 identical Y chains  can pass through human placenta  Is a secondary immune response

6.  IgA:  H chain, J chain, L chain, secretory component  Mucosal immunity

8.  5 classes of immunosuppressant drugs 1)glucocorticoids 2)cytostatics 3)antibodies 4)drugs acting on immunophilins 5)other drugs5)other drugs.

9.  Used to prevent rejection of transplanted organs  Auto immune disease  Chronic anti-inflammatory

10.  Apheresis: the process by which plasma or cellular components are separated from the whole blood

11.  Plasmapheresis DFPP: apheresis used to remove blood plasma. In DFPP, plasma is separated with a plasma separator and passes through the plasma component separator with a small pore size. Large molecular-weight proteins are discarded and small molecular-weight substances including valuable albumin are returned to the patient. Small amount of substitution fluid such as albumin may be added.

13.  Plasma exchange: Plasma exchange involves replacing the plasma (the liquid part of a person’s blood) with plasma from a donor.

14.  In PA, plasma is separated with a plasma separator and flows into a plasma adsorption column.  Pathogenic substances are adsorbed and removed due to affinity between ligands and pathogenic substances. Note: Fluids are not replaced

16.  Patient 1  After three successive apheresis treatments  Vertical line after transplant occurs  Antibody production suppressed

17.  Patient 3  Received extended apheresis treatment  IgM shows typical response  IgG and IgA show increased immune response

19.  PA more consistent with each antibody type  IgM moves consistently with each apheresis method  IgG and IgA differ amongst apheresis types  PE clears IgG fastest, PA clears it slowest  DFPP clears IgA fastest, PA clears it slowest  Can customize apheresis method depending on patient’s condition

20.  Examines the effectiveness of certain immunosuppressive procedures  Enables clinicians to better categorize the patient’s response to implants  Enables clinicians to estimate antibody production during transplant procedures using algorithm

21.  No data post-operatively for the patient in figure 3.  Plasmapheresis used in unequal proportion compared to the two other methods of apheresis.  Limited sample size  Quantity and type of immunosuppressive drugs used was not recorded