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Published bySheena Todd Modified over 9 years ago
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TOPIC: Microscope AIM: What are the parts of a microscope?
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Microscope Instrument that produces a larger image of an object (specimen) using lenses
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Lens Piece of curved glass
Causes light rays to come together or spread apart Magnify an image
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Uses 1 lens and light to magnify the specimen
Simple Microscope Uses 1 lens and light to magnify the specimen
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Uses 2 lenses & light to magnify specimen Need stain (dye) to see it
Compound Light Microscope Uses 2 lenses & light to magnify specimen Need stain (dye) to see it
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Blood
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Cheek Cells 40x
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Onion Cells 10x
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PARTS OF A MICROSCOPE
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1. eyepiece Ocular lens (usually 10x) Lens you look through
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2. Objective lenses Contains lenses Low, medium, high power High Power
Low Power High Power
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Low power objective = used to locate the specimen on the slide
Larger field of view (See more of the entire object) High power objective = More magnification Smaller field of view(see less of the entire object)
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Holds & rotates obj. lenses
3. Nosepiece Holds & rotates obj. lenses
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Connects eyepiece to nosepiece
4. Body Tube Connects eyepiece to nosepiece
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Supports body tube 5. Arm
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Bottom = support 6. Base
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Where slide is placed 7. Stage
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Hold slide in place 8. Stage Clips
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Illuminates specimen, provides light
9. Mirror/ light
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Controls the AMOUNT of light used
10. Diaphragm Controls the AMOUNT of light used
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Larger focusing knob 11. Coarse Adjustment Knob
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For focusing under LOW POWER ONLY
If used under high power, objective lens and/or slide can break
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Focusing for High Power
Sharpens image Focusing for High Power 12. Fine Adjustment Knob
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Most powerful microscope Electron microscope: magnifies up to 1 million times Uses beam of electrons instead of light
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Fly
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Spider
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Termite head
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Tapeworm
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Blood
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SEM Scanning Electron Microscope Produces real, 3D image
Only scans surface magnifies 20x - to 200,000x SEM
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TEM Transmission Electron Microscope
Produces 2D image of thinly sliced organism magnifies 1,000x -1,000,000x
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View living specimens without staining
Phase Contrast Microscope View living specimens without staining
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Light microscope Phase contrast microscope
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