1. Overview

2. DNA (Deoxyribonucleic Acid)

3. What is DNA? Deoxyribonucleic acid contains all the genetic information for living organisms. It is a very long molecule of repeating units called nucleotides, which contain nitrogenous bases of: adenine (A), guanine (G), cytosine (C), and thymine (T)

5. Genetic material of cells… GENES – units of genetic material (DNA or RNA) that CODE FOR A SPECIFIC TRAITGENES – units of genetic material (DNA or RNA) that CODE FOR A SPECIFIC TRAIT DNA and RNA are NUCLEIC ACIDSDNA and RNA are NUCLEIC ACIDS DNA/RNA is made up of repeating molecules called NUCLEOTIDESDNA/RNA is made up of repeating molecules called NUCLEOTIDES

6. DNA Nucleotide O O=P-O OPhosphate Group Group N Nitrogenous base (A, G, C, or T) (A, G, C, or T) CH2 O C1C1 C4C4 C3C3 C2C2 5 Sugar Sugar(deoxyribose)

7. DNA Double Helix Nitrogenous Base (A,T,G or C) “Rungs of ladder” “Legs of ladder” Phosphate & Sugar Backbone

8. DNA Double Helix P P P O O O 1 2 3 4 5 5 3 3 5 P P P O O O 1 2 3 4 5 5 3 5 3 G C TA

9. Nitrogenous Bases PURINESPURINES Adenine (A) 1.Adenine (A) Guanine (G) 2.Guanine (G) PYRIMIDINESPYRIMIDINES Thymine (T) 3.Thymine (T) Cytosine (C) 4.Cytosine (C) T or C A or G

10. Chargaff’s Rule Adenine ThymineAdenine must pair with Thymine GuanineCytosineGuanine must pair with Cytosine about the sameTheir amounts in a given DNA molecule will be about the same. G C TA

11. BASE-PAIRINGS CG H-bonds T A

12. Genetic Diversity… Different arrangements of NUCLEOTIDES in a nucleic acid (DNA) provides the key to DIVERSITY among living organisms.Different arrangements of NUCLEOTIDES in a nucleic acid (DNA) provides the key to DIVERSITY among living organisms.

13. The Code of Life… The “code” of the chromosome is the SPECIFIC ORDER that bases occur.The “code” of the chromosome is the SPECIFIC ORDER that bases occur. A T C G T A T G C G G…

14. DNA is wrapped tightly around small proteins called histones and coiled tightly to form chromosomes See p. 297

15. How do we pass this genetic code to our own cells our offspring? DNA must be replicated before cells divide during mitosis or meiosis… What stage is DNA replicated in before cell division?

16. DNA Replication DNA must be copiedDNA must be copied The DNA molecule produces 2 IDENTICAL new complementary strands following the rules of base pairing:The DNA molecule produces 2 IDENTICAL new complementary strands following the rules of base pairing: A-T, G-C Each strand of the original DNA serves as a template for the new strand See p. 298Each strand of the original DNA serves as a template for the new strand See p. 298

17. DNA Replication Semiconservative Model:Semiconservative Model: 1.Avery-McCarty-McLeod 2.Watson and Crick showed: 2.Watson and Crick showed: the two strands of the parental molecule separate, and each functions as a template for synthesis of a new complementary strand.. Parental DNA DNA Template New DNA

19. DNA Replication Each strand gets a new complimentary strand, synthesized from free-floating nucleotides. Each strand is synthesized in the 5’ to 3’ direction; one strand made in one direction and other strand in opposite direction (leading vs. lagging strands) Two identical strands of DNA are made http://www.veoh.com/browse/videos/category/educational/watch/v1011706jGF8tJBF#

20. Enzymes Involved in DNA Replication Helicase: Unzips DNA by breaking the hydrogen bonds between the nitrogenous bases Primase: Attaches RNA primer to DNA strand DNA Polymerase III: Makes the new strand of DNA by adding DNA nucleotides DNA Polymerase I: Replaces RNA primer with DNA nucleotides Ligase: Seals the gap between Okazaki Fragments

21. 1. Why is replication necessary? 2. When does replication occur? 3. Describe how replication works. 4.Use the complementary rule to create the complementary strand: A--- ? G--- ? C--- ? T--- ? A--- ? G--- ? A--- ? G--- ? C--- ? A--- ? G--- ? T--- ? Replication Quiz

22. 1. Why is replication necessary? So both new cells will have the correct DNA 2. When does replication occur? During interphase (S phase). 3. Describe how replication works. Enzymes unzip DNA and complementary nucleotides join each original strand. 4. Use the complementary rule to create the complementary strand: A--- T G--- C C--- G T--- A A--- T G--- C A--- T G--- C C--- G A--- T G--- C T--- A Replication Quiz

23. (1961) Watson & Crick proposed… …DNA controlled cell function by serving as a template for PROTEIN structure.…DNA controlled cell function by serving as a template for PROTEIN structure. 3 Nucleotides = a triplet or CODON3 Nucleotides = a triplet or CODON (which code for a specific AMINO ACID) See p.303 AMINO ACIDS are the building blocks of proteins.AMINO ACIDS are the building blocks of proteins.

24. DNA Transcription DNA can “unzip” itself and RNA nucleotides match up to the DNA strand.DNA can “unzip” itself and RNA nucleotides match up to the DNA strand. Both DNA & RNA are formed from NUCLEOTIDES and are called NUCLEIC acids.Both DNA & RNA are formed from NUCLEOTIDES and are called NUCLEIC acids. See p.301

25. DNA Translation The cell uses information from “messenger” RNA to produce proteinsThe cell uses information from “messenger” RNA to produce proteins See p.304-305 We will discuss details of this on a later date

26. Transcription/Translation Quiz 1.Why is transcription necessary? 2.Describe transcription. 3.Why is translation necessary? 4.Describe translation. 5.What are the main differences between DNA and RNA. 6.Using the chart on page 303, identify the amino acids coded for by these codons: UGGCAGUGC

27. 1. Why is transcription necessary? Transcription makes messenger RNA (MRNA) to carry the code for proteins out of the nucleus to the ribosomes in the cytoplasm. 2. Describe transcription. RNA polymerase binds to DNA, separates the strands, then uses one strand as a template to assemble MRNA. 3. Why is translation necessary? Translation assures that the right amino acids are joined together by peptides to form the correct protein.

28. 4. Describe translation. The cell uses information from mRNA to produce proteins. 5. What are the main differences between DNA and RNA. DNA has deoxyribose, RNA has ribose; DNA has 2 strands, RNA has one strand; DNA has thymine, RNA has uracil. 6.Using the chart on page 303, identify the amino acids coded for by these codons: UGGCAGUGC tryptophan-glutamine-cysteine

29. AMAZING DNA FACTS… DNA from a single human cell extends in a single thread for almost 2 meters long!!!DNA from a single human cell extends in a single thread for almost 2 meters long!!! It contains information equal to some 600,000 printed pages of 500 words each!!!It contains information equal to some 600,000 printed pages of 500 words each!!! (a library of about 1,000 books)

30. LET’S REVIEW DNA… LM p.44 1.List the conclusions Griffith & Avery, Hershey & Chase drew from their experiments. 2.Summarize the relationship between genes & DNA. 3.Describe the overall structure of the DNA molecule. 4.What are the 4 kinds of bases?