1. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 1 Notice : This technical data is furnished on the condition that it will be used by and disclosed to the receiving Cooperating agency and its contractors and sub contractors only for the purposes of fulfilling the cooperating agency’s responsibilities under the Space Station Intergovernmental Agreement (IGA) and Memorandum of Understanding (MOU). It shall not be used for any other purpose, nor disclosed or retransferred to any other entity or government without prior written permission of the Japan Aerospace Exploration Agency (JAXA). Saibo Rack Maintenance JEM SAIBO MAINT 31407 Instructor:Takashi Yamasaki Toru Mori (yamasaki.takashi@jaxa.jp) (mori.tooru@jaxa.jp)

2. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 2 Objectives  Saibo Rack maintenance overview  Key points during Saibo Rack maintenance operations  Saibo Rack Maintenance Operations List two objectives of Saibo Rack maintenance operations Explain which equipment crew must attach to CB before turning on CB UV Lamps to prevent electro magnetic interference. Explain which equipments crew must not touch in CB Operation Chamber to prevent crew exposure to broken glass. Perform Saibo Rack maintenance operations according to ODFs without instructor’s assistance. After this lesson the trainee will be able to:

3. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 3 Lesson Outline Contents: - Saibo Rack Maintenance Overview - Key Points during CB Maintenance Operations - Key Points during CBEF Maintenance Operations - Appendix Page: 4 6 7 8

4. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 4 CBEF Clean Up CBEF Function Checkout CB Activation CB Function Checkout CB Deactivation CB Stow CB Setup :Crew operations :Ground operations LEGEND: :Crew/Ground cooperative operations Saibo Rack Maintenance Overview - Function Checkout - Clean Up Objectives of Saibo Rack maintenance Saibo Rack maintenance operations flow CB Clean Up CB Stow CB Setup Clean CB and Valve Check Video Cable Installation CBEF Activation Canisters Installation Function Checkout CBEF Deactivation Canister Retrieval Video Cable Stow Clean CBEF Incubators Clean Bench Maintenance Operations Cell Biology Experiment Facility Maintenance Operations

5. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 5 Saibo Rack Maintenance Overview Equipments found in Saibo Rack Maintenance Operations  CB Shield - Prevent Electro Magnetic Interference while UV Lamps are ON. (- Block JEM Cabin lights to make fluorescence observation efficiently.)  CB Relief Valve - Release air in CB Air-tight Chambers if JEM cabin should depressurize.  BZK Wipe - Benzalkonium chloride antiseptic towelettes are alcohol-free wet wipes used for general antiseptic cleansing.  Meas Exp Unit B (CBEF Canister) - Canister is a container of samples and equipments.

6. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 6 Key Points during CB Maintenance Operations CategoryEquipmentKey Points Crew Safety Excessive EMI Radiation ShieldInstall CB Shield before using UV Lamps to prevent Electro Magnetic Interference. Crew entrapmentAir-tight Chamber When CB is in ‘Stowed Configuration’ fill gaps between the OC and Outer Chassis with Gap Protectors to prevent crew entrapment. Crew Exposure to Broken Glass Operation Chamber Verify no broken glass in Operation Chamber before opening OC front panel. OC Lamp, UV Lamps Do not touch OC Lamp or UV Lamps with bare hand. Oil from hands may cause breakage. Time Critical OperationN/A Dos and Don’tsAir-tight Chamber Pull out/stow Air-tight Chamber slowly at about 10 cm per minute to avoid damage to bellows. OC CameraRaise OC Camera (Lens and Flexible Arm) above the inner plate before stowing the Operation Chamber. Difficulty in OpsN/A Possible Mis-OpsN/A NOTE: Underlined key points have been covered by CB Advanced Training.

7. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 7 NOTE: Underlined key points have been covered by CBEF Advanced Training. CategoryEquipmentKey Points Crew Safety Electrical ShockSample Tray, Centrifuge Before attaching/detaching the Canisters to/from the Sample Tray/ Centrifuge, turn power to Canister off by switches on the Sample Tray/Centrifuge. Entanglement with rotating device Incubator Unit 1G Verify visually through the Observation Window that the Centrifuge is not rotating before opening the door of the Incubator Unit 1G. CentrifugeWhen attaching/detaching the Canisters to/from the 1G Centrifuge, lock turntable of the Centrifuge. Time Critical OperationN/A Dos and Don’tsIncubator UnitDo NOT touch inside Incubator Unit with bare hand to keep it clean. Sample Tray, Centrifuge Be careful not to pinch fingers when releasing drawer locks of slide rails on Sample Tray/Centrifuge. Difficulty in OpsN/A Possible Mis-OpsN/A Key Points during CBEF Maintenance Operations

8. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 8 Appendix - CB Gap Protector - CB UV Lamp Illumination - CB Software Modes - CB Schematics - Meas Exp Unit B - Meas Exp Unit B Schematic - Acronyms

9. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 9 CB Gap Protector

10. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 10 CB UV Lamp Illumination This is a not real situation. Before turning on UV Lamps, install CB Shield to prevent Electro Magnetic Interference. NOTE:

11. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 11 Standby Mode Start up Mode Main Startup Mode Standby Mode Main Control Mode Main Environment Set Up UV Sterilization UV Sterilization Mode Adsorption DC DC Gas Processing Mode Ventilation OC OC Ventilation Mode Exhaust OC OC Exhaust Mode Control Mode Adsorption OC OC Gas Processing Mode Microscope Control Telescience Control Telescience Control Mode Microscope Control Mode Microscope Set Up (*1) (*1): Only in Telescience Control Mode, ground commands become enable. CB Software Modes

12. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 12 CB Schematics

13. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 13 CB Schematics (Cont’d)

14. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 14 CB Schematics (Cont’d)

15. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 15 CB Schematics (Cont’d)

16. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 16 CB Schematics (Cont’d)

17. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 17 CB Schematics (Cont’d)

18. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 18 Meas Exp Unit B

19. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 19 Meas Exp Unit B Schematics Meas Exp Unit B

20. Training Purposes Only JEM SAIBO MAINT 31407 JMU-081195 (Ver. 1.0) 20 Acronyms BZK Wipes CB CBEF EMI OC ODF UV Benzalkonium Chloride Antiseptic Towelettes Clean Bench Cell Biology Experiment Facility Electro Magnetic Interference Operation Chamber Operations Data File Ultra Violet