1. 5 15 25 20 10 Ct value Log concentration miR-375 miR-221 Supplementary Figure S1

2. Supplementary Figure S2 T-Bil CA19-9 AMY miR-221 concentration (amol/μl)

3. VariablesPatients (n=47) miR-221 Concentration (amol/μ ｌ ) p-value a Age (year)mean (range)67 (42-84) ≤6516( 34.0% )3.31E-02 0.509 ≥6631( 66.0% )4.29E-02 Sex Male 27( 57.4% )4.10E-02 0.508 Femal20( 42.6% )4.29E-02 Histopathology Adenocarcinoma 42( 89.4% )4.38E-02 0.359 Others5( 10.6% )2.56E-02 Location Ph34( 72.3% )4.15E-02 0.537 Pb/Pt13( 27.7% )3.44E-02 Depth of invasion T0/1/27( 14.9% )2.87E-02 0.518 T3/440( 85.1% )4.17E-02 Lymph node metastasis Negative17( 36.2% )3.71E-02 0.233 Positive30( 63.8% )4.27E-02 Distant metastasis Negative38( 80.9% )3.42E-02 0.023 Positive9( 19.1% )6.08E-02 Stage 0- Ⅱ A 16( 34.0% )3.54E-02 0.117 Ⅱ B- Ⅳ 31( 66.0% )4.23E-02 Treatment Resectable37( 78.7% )3.47E-02 0.030 Non-resectable10( 21.3% )5.75E-02 Characteristics and plasma miR-221 concentrations of 47 consecutive PCa patients NOTE. Significant values are in boldface type. a P values are from the Mann-Whitney U-test and were considered significant at 0.05. Supplementary Table S1

4. Supplementary Table S2 Plasma miR-221 concentration (amol/μl) SamplenMean95% CI b p-value c Pancreatic cancer470.03960.0287-0.0505p < 0.001 Benign tumor a 90.01610.0094-0.0227p = 0.539 Normal control300.01580.0113-0.0202 a Five IPMNs, 2 serous cystadenomas, and 2 pancteatitis tumors were included in benign tumors. b 95% confidential interval c Plasma concentrations of each group were compared with those of normal controls by Mann-Whitney U test. Differential expression pattern of plasma miR-221 in patients with pancreatic, pancreatic benign tumors, and normal controls

5. Plasma miR-221Tumor miR-221 High plasma miR-221 cases a Concentration (amol/μl)Tumor expressionmiR-221/U6B ratio 11.94E-01high19.10 21.34E-01high1.20 37.16E-02high2.83 46.09E-02high3.39 55.58E-02high1.51 64.93E-02high1.34 Rate reflecting tumor dynamics100% b a Cut-off value of plasma miR-221 concentrations in healthy volunteers (mean+2SD): 4.047E-02 amol/μl b Cut-off value of miR-221/U6B ratios in normal pancreatic tissues (median): 1.00 Relationship between miRNA-221 in plasma and pancreatic cancer tissues Supplementary Table S3