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Unique Microplates for
UVStar® Unique Microplates for the UV-Range
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Pre-UVStar®-Period At my time, only quartz glass was
available, and it was fragile expensive Microplates hard to clean for reuse limited in throughput
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Uniqueness of UVStar® Microplates with outstanding optical properties
Dedicated for OD reading in the UV-range Proprietary, unique resin Optical window down to 200 nm Available in 96 and 384 well format
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Prerequisites for UVStar®
Availability of a unique proprietary plastic Material free of any UV-blocking additives Production of high quality films (140 µm) Lamination process free of glue and solvents Adaptation of µClear®-process to UVStar® UVStar_Rev. 1
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µClear®-Technology Direct lamination technology
Process free of glue or solvents µm films Enables multi-mode detection Worldwide Patents Germany: DE Europe: Pat. pending USA: Pat. pending
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Applications of UVStar®-Plates
Optical window down to 200 nm Background: < nm 260 nm Purity of 260/280 nm Protein 280 nm
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Absorbance of DNA vs. Protein
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Clear Bottom Plates 1 mm bottom 140 µm bottom bonding of glass sheets
bonding of plastic films 2 component injection molding
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Advantages of µClear®-Technology
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From µClear® to UVStar®
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Absorbance of UV-transparent Resins Greiner vs. Competitors
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UV-Light Absorbance 384 well µClear® vs. UVStar®
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Measurement of DNA-Conc. 96 vs. 384 Well UVStar®-Plates
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Cell Growth (WST) in TC-treated 384 Well UVStar®-Plates
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Growth of CHO-Cells in TC-treated 96 Well Plates
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Homogeneity of 384 Well Plates
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Performance of Background 384 Well UVStar® @ 260 nm
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Drift and Background Greiner vs. Competitor
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Drift and Background Greiner vs. Competitor
Mean 0,0524 Standard Deviation 0,002 Correlation of Variation 4 % Mean 0,0498 Standard Deviation 0,001 Correlation of Variation 2 %
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Pathlength & Lambert Beer’s Law I = Io ·e-k’ · c · x
Well pathlength [x]: not defined Cuvette pathlength [x]: fixed
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Correlation Pathlength vs. Absorption
@ 260 nm Quartz Cuvette [10 mm] = 0,08 OD UVStar_Rev. 1
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Chemical and Thermal Resistance
UVStar_Rev. 1
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Benefits of UVStar®-Plates
Available in a 96 well and 384 well format Extended optical window from 200 to 1000 nm Background less than nm Minimized variation from well to well Ideal for quantifying DNA and total protein No more need for expensive/fragile quartz plates
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UVStar® Made by
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