1. DNA for Dummies (and the intellectually astute)
A Dr. Parrott Production

2. Secret of Photo 51: What organic polymer? Made of what monomers?
Components?
Tour of the Basics

3. DNA Wrap Basic
DNA Wrap Advanced
DNA Coiling

4. Replication: DNADNA
Occurs during S phase of interphase in reproducing cells only
DNA template is copied complimentarily and semi conservatively
Occurs only in the nucleus

5. Meselson-Stahl Experiment How DNA Replicates DNA Replication
Bidirectional Replication Replication Complete General DNA Workshop

6. Make a DNA strand complimentary to the following:
DNA1: ATG GTG CAC CTG ACT CCT GTG GAG AAG TCT
DNA2: TAC
mRNA:
Amino Acids:
tRNA:
replication

7. DNA Replication Basic DNA Replication Fork Rep Fork 2
DNA Replication DNA Polymerase

8. Tran scription: DNAmRNA
Occurs continually throughout the cell cycle with the exception of mitosis
Occurs in the nucleus as messenger RNA copies DNA

9. RNA Splicing Transcription Transcription Complete
Transcription the Movie Regulated Transcription

10. Transcribe the above DNA template into an mRNA:
Make a DNA strand complimentary to the following:
DNA1: ATG GTG CAC CTG ACT CCT GTG GAG AAG TCT
DNA2: TAC
Transcribe the above DNA template into an mRNA:
mRNA: UAC
Amino Acids:
tRNA:
transcription

11. Translation: mRNAprotein
Occurs continually throughout the cell cycle
Occurs at the ribosome as it
reads the mRNA codons
Matches codons to amino acids
Prompts tRNA to bring a.a.
Attaches a.a. with peptide bonds
DNA to Protein 2
PP Synthesis Walkthrough

13. Translation tRNA anticodon is complimentary to mRNA codon
tRNA is specific to the amino acid it brings
Peptide bonds connect amino acids to form polypeptide (protein)
Translation Basic Translation Advanced Protein Synthesis
Protein Synthesis 2 Translation the Movie

14. Protein Synthesis = Transcription + Translation = DNA  proteins

15. Make a DNA strand complimentary to the following:
DNA: ATG GTG CAC CTG ACT CCT GTG GAG AAG TCT
DNA: TAC
mRNA (codons): UAC
Translate the above mRNA strand into an amino
acid sequence (protein)
Amino Acids: Tyrosine
tRNA (anticodons):
translation

16. Watch RNAi Online: http://www. pbs. org/wgbh/nova/sciencenow/3210/02

17. Make a DNA strand complimentary to the following:
DNA: ATG GTG CAC CTG ACT CCT GTG GAG AAG TCT
DNA: TAC
mRNA (codons): UAC
Amino Acids: Tyrosine
What are the anticodons on the tRNA for the above strands?
tRNA (anticodons): AUG

18. To summarize…
Protein Synthesis

20. Mistakes/Mutations
Mutation any permanent alteration in the sequence of DNA bases
Mutagen causes a mutation i.e. chemicals, radiation, spontaneous
Point mutations can result in frameshifts in the reading frame
Chromosomal mutations can result in translocations
Immortal Thread

21. Mistakes/Mutations
Silent mutation change in n.t. sequence, but no change in product
Missense mutation swapping of a.a. (sickle cell anemia, PKU)
Nonsense mutation insertion of stop codon into sequence
Mutations & Sickle Cell, 2
How Cancer Grows, 2
Evolutionary Arms Race: HIV Resistance, 2 G
Silent Mutation

22. DNA Technology & Applications
What is the GPS & element?
Medicine
Agriculture
Forensics
What are some uses?
Finding Disease Genes
Gene therapy
Drugs
Biological Warfare
Genetic Testing Ethics
GMOs
The Right to Know
A Silent Forest
Classical vs Transgenic Breeding
Bioremediation
Biofuels
Crime Scene Investigation
Genealogy
What are some examples
Bioengineering Body Parts
RNAi Therapy
Breast Cancer
Alzheimer's Disease
Plasmids & Protein
Bt Corn
Super Salmon, 2
Harvest of Fear
Babies by Design?
Transgenic Mouse
The Killer’s Trail
Forensic Anthropology: Immigration
Laetoli Footprints
What are some pros?
What are some cons?

23. Resources DNA Learning Center: 3D Animations Genetics Movies:
DNA Tutorial:
Bacterial Replication Animations:
Telomere Synthesis (shortening of linear chromosomes):
Gene Processing
Replication Animations:
Gene Expression Animations:
Gene Technology:
DNA the Secret of Life Video
Cracking the Code of Life Video
Beadle-Tatum Experiments (one gene, one pp)
Protein Synthesis Tutorial