1. Announcements Please remove exam answer sheets, homework from your folders. News story on “semi-identical twins” posted on Blackboard. Today, April 4: microtubules, pp. 425-437 Friday, April 6: Microtubules and microfilaments, pp. 425-446 (742-762 in 5 th Ed.) Monday, April 9: Muscle contraction, Cell motility, intermediate filaments.

2. Exam 3 Results Key and full standings posted outside BR 179 Average before curve = 45/72 = 63% Average after curve = 58/72 = 80% –13 points added to everyone’s raw score to curve High = 87/72 (120%) 90-100% (>=65): 16 80-89% (58-64): 7 70-79% (50-57): 9 60-69% (43-49): 9 <60% (<43): 5 Do add up your raw score, count the number correct, multiply by 3, add any extra credit then add 13 points.

3. Outline/Learning Objectives I. Microtubules A.Structure and assembly B.Dynamic instability model C.Microtubules in motion II. Microfilaments A.Video of MF- based motility B.Actin and assembly After reading the text, attending lecture, and reviewing lecture notes, you should be able to: Compare and contrast microtubules, microfilaments and intermediate filaments. Describe microtubule structure and assembly/disassembly, including nucleation and dynamic instability. Explain the function of MAPs, kinesin, and dynein. Explain how axonemal structure determines ciliary movement. Describe microfilament structure and assembly. Give examples of microfilament- based motility.

4. Semi-identical twins Souter et al. 2007

5. RSTK Pathway Another class of growth and differentiation factors signal through receptor serine- threonine kinases. E.g. TGFβ Many involved in development Smad transcription factors become active when phosphorylated.

7. Steps of apoptosis, or programmed cell death Examples: normal cell senescence, damaged/infected cells, embryonic digits, tadpole tails, pruning of infant neurons.

8. Apoptosis Pathway Programmed cell death discovered in nematode C. elegans. Present in other organisms, e.g. mammals. Triggered either by presence of death factors or removal of survival factors. (a) killer lymphocytes acting on infected cells (b) removal of survival factors/ DNA damage

9. Components and Functions of the Cytoskeleton Recent review: Pollard, T.D. (2003). Nature 422:741-745.

10. Self-assembly of cytoskeletal filaments Polymerization does not involve covalent bonds, rather weak interactions between monomers. –Therefore, can assemble or disassemble rapidly, without covalent bond formation or breaking. Combine strength with adaptability through lateral interactions in addition to length-wise interactions. Nucleation is rate-limiting step of polymerization

11. Microtubules Cytoplasmic MTs: –dynamic growth and collapse –in nerve processes, mitotic spindles, elongating cells, etc. Axonemal MTs: –stable –in cilia, flagella http://raven.zoology.wa shington.edu/celldynami cs/http://raven.zoology.wa shington.edu/celldynami cs/

12. Videos: Microtubules in Motion 1.Interphase microtubules: dynamic instability 2.Chromatophores: pigment granule transport along microtubules 3.Axopodia in Heliozoans: prey capture and transport along microtubules 4.Mitosis in animal, plant cells

13. Microtubule Structure 25 nm outer diameter 12 protofilaments, polymers of heterodimeric  and  -tubulin (50 kD each). –  -tubulin binds GTP Destabilized by: –cold, pressure –Drugs colchicine, nocodazole, etc. –> 10 -6 M Ca 2+ Stabilized by: –Drug taxol –MT-associated proteins (MAPs)  

14. Centrosomes organize cytoplasmic MTs Animal centrosomes contain two centrioles (9 short triplet MTs), plant centrosomes don’t. Pericentriolar material nucleates MTs (animals and plants) –  (gamma)-tubulin –pericentrin Minus end of MT starts in centrosome, plus end is away.

15. Microtubule organization in cells

16. Dynamic Instability of MTs: GTP cap stabilizes + end of MTs Recent review: Howard and Hyman. (2003). Nature 422:753-758.

17. Microtubule Poisons (and Anti-Cancer Drugs) Colchicine –Alkaloid from meadow saffron –Binds tubulin monomers, prevents assembly  processes that require assembly are blocked –Colcemid, nocadazole, etc. also (-) assembly Taxol –Alkaloid from Pacific yew tree, effective chemo- in ovarian cancer –Can now be synthesized in vitro –Binds microtubules, stabilizes, prevents disassembly  processes that require disassembly are blocked