1. 5.1 Proteomics tools on ExPASy

2. 5.2 (Part 1) Primary, secondary, and tertiary protein structure

3. 5.2 (Part 2) Primary, secondary, and tertiary protein structure

4. 5.3 (Part 1) Protein structural profiles

5. 5.3 (Part 2) Protein structural profiles

6. 5.3 (Part 3) Protein structural profiles

7. 5.4 (Part 1) Two-dimensional polyacrylamide gel electrophoresis

8. 5.4 (Part 2) Two-dimensional polyacrylamide gel electrophoresis

9. 5.5 Affinity chromatography

10. 5.6 (Part 1) Basis of mass spectrometry peptide sequencing

11. 5.6 (Part 2) Basis of mass spectrometry peptide sequencing

12. 5.6 (Part 3) Basis of mass spectrometry peptide sequencing

13. 5.7 (Part 1) Peptide sequencing by tandem mass spectrometry

14. 5.7 (Part 2) Peptide sequencing by tandem mass spectrometry

15. 5.8 (Part 1) Quantitative proteomics using ICAT reagents

16. 5.8 (Part 2) Quantitative proteomics using ICAT reagents

17. 5.8 (Part 3) Quantitative proteomics using ICAT reagents

18. 5.8 (Part 4) Quantitative proteomics using ICAT reagents

19. 5.9 (Part 1) Antibodies and immunohistochemistry

20. 5.9 (Part 2) Antibodies and immunohistochemistry

21. 5.10 Protein microarrays

22. 5.11 Self-assembling NAPPA protein microarrays

23. 5.12 (Part 1) Yeast two-hybrid (Y2H) screens

24. 5.12 (Part 2) Yeast two-hybrid (Y2H) screens

25. 5.13 Applications of structural proteomics

26. 5.14 Flow diagram for solving a protein structure

27. 5.15 The Drosophila Engrailed homeodomain dimer bound to DNA

28. 5.16 (Part 1) Fold recognition

29. 5.16 (Part 2) Fold recognition

30. 5.17 Frequency of mutations recovered in “saturation” screens

31. 5.18 (Part 1) The three major types of mutagen

32. 5.18 (Part 2) The three major types of mutagen

33. 5.18 (Part 3) The three major types of mutagen

34. 5.19 (Part 1) F 1 and F 3 genetic screens

35. 5.19 (Part 2) F 1 and F 3 genetic screens

36. 5.20 (Part 1) Targeted mutagenesis

37. 5.20 (Part 2) Targeted mutagenesis

38. 5.21 (Part 1) Inhibitory RNA expression (RNAi)

39. 5.21 (Part 2) Inhibitory RNA expression (RNAi)

40. 5.22 Targeted loss- and gain-of-function genetics

41. 5.23 Deficiency mapping

42. 5.24 Transposon mutagenesis and enhancer trap screens

43. 5.25 Floxing mice

44. 5.26 Genetic fingerprinting

45. Box 5.1 (Part 1) Hidden Markov Models in Domain Profiling

46. Box 5.1 (Part 2) Hidden Markov Models in Domain Profiling

47. Box 5.2 (Part 1) Biological Networks In Genome Science

48. Box 5.2 (Part 2) Biological Networks In Genome Science

49. Box 5.2 (Part 3) Biological Networks In Genome Science

50. Box 5.2 (Part 4) Biological Networks In Genome Science

51. Box 5.3 (Part 1) Transgenic Animals and Plants

52. Box 5.3 (Part 2) Transgenic Animals and Plants

53. Exercise 5.1 Structural annotation of a protein

54. Exercise 5.2 (Part 1) Identification of a protein on the basis of a MS profile

55. Exercise 5.2 (Part 2) Identification of a protein on the basis of a MS profile

56. Exercise 5.3 Formulating a network of protein interactions

57. Exercise 5.4 Designing a genetic screen