1. Genetic Enhancement of Biodiesel Feedstocks Richard F. Wilson Oilseeds & Bioscience Consulting, LLC Raleigh, NC

2. Energy Costs PetroleumBiofuel

3. Vegetable Oil Prices (¢/lb) Oil 2002 Current Peanut32.565.6 Sunflower23.358.1 Cotton18.046.6 Corn19.243.6 Canola20.547.7 Soybean16.543.5 Palm14.936.2 USDA FAS October 2007

4. World Vegetable Oil Situation 2006/2007 MMT MMT/yr Supply 132 + 5.5 Demand 122 + 5.3 End Stocks 10 10 MMT Oil represents about 28 days supply World Use of Diesel Fuel = 321 billion gallons in 2007 Need 300 MMT of vegetable oil to make B20 Biodiesel USDA FAS Sept 2007 Growth Rate

6. How to Ensure Adequate Supply of Vegetable Oils? Increase Crush Capacity Increase Area of Production Increase Yield per Area Develop New Oil Crops Genetically Enhance Oil Concentration & Quality in Major Oilseed Commodities

7. Variation in Oil Concentration Soybean 7.0 to 26% Sunflower 9.0 to 53% Canola 12 to 42% Cotton 20 to 30% Peanut 30 to 65% An increase from 20 to 30% oil in soybean seed equals a 50% gain in oil content per bushel

8. Mapping & Marking Oil Genes

9. Developing New Genomic Breeding Tools New DNA markers in Physical Map Candidate Genes Genetic markers Genetic map QTL BAC contigs, ESTs Minimal Tiling Path & BAC-end sequences Physical Map Bioinformatic Analyses

13. Genetic Regulation of Oil Concentration

14. High Yield x High Oil ↓ F 1 Plants ↓ F 2 Population ↓ SSD F 3 Population ↓ SSD F 4 Population ↓ Single Plants F 4:5 Families ↓ Select & Bulk Test for Yield & Oil ↓ Retest selections Breeding for Increased Oil Concentration & Yielding Ability

15. Genetic Enhancement of Oil % World Oil Supply, USDA FAS, Sept 2007

16. Which one is Crude Peanut Oil?

17. Oleic acid improves oxidative stability and ignition properties (cetane index) of biodiesel

18. KAS II FAT B FAD 2 FAD 3 D9 DS

19. Linolenic Acid Pathway

20. Genetic Regulation of Oleic Acid Concentration in Soybean

21. MAS with Perfect Gene Markers 1% 18:3 GMFAD3A has 2 recessive alleles (aa 29, aa cx ) GMFAD3B has 1 recessive allele (bb 29 ) GMFAD3C has 2 recessive alleles (cc cx, cc 29 ) aa 29 bb 29 cc 29 X aa cx BB cc cx F1 F2 aa 29 bb 29 cc cx aa cx bb 29 cc 29 ~1% of the F3 generation 3% 18:3 Kristen Bilyeu, Columbia MO

22. New Sources of Oleic Acid