1. 1 High Hydrostatic Pressure (HHP) Technology presentation

2. 2Introduction The High Hydrostatic Pressure (HHP) treatment is an athermic decontamination process which consists in subjecting packaged food to water pressures from 100 to 900 MPa. The pressure applied is isostatically transmitted inside a pressure vessel.

3. 3 Diagram of the HHP equipment

4. 4 Food that can be HHP treated Solid foods, mainly vacuum packed - Dry-cured or cooked meat products - Cheeses - Fish, seafood, marinated products - Ready to eat meals, sauces - Fruits, marmalades / jams - Vegetables Liquid foods, in flexible packaging - Dairy products - Fruit juices - Nutraceutical formulations

5. 5 Food that can not be HHP treated Solid foods with air included - Bread - Mousse Packaged foods in completely rigid packaging - In glass - Canned Foods with a very low water content - Spices - Dry fruits

6. 6 Main technological effects of HHP in meat products About color: – In fresh or marinated meat, the iron in the myoglobin changes from ferrous to ferric and globin is denatured: the red color is lost About texture: – Inhibition or stimulation of the proteolytic activity in musclesactivity muscles (depending on processing conditions) – Proteins are partially denaturized in products where proteins have not been previously modified by other process: heating, drying, fermentation … About fats and lipids: – Reversible crystallization

7. 7 Treated beef samples (1) Top view of the fresh and frozen beef samples treated by HHP

8. 8 Treated beef samples (2) Cooked samples: view from the top (a); view of the inside (b)

9. 9 Treated commercial beef products samples Commercial beef products before and after HHP treatment

10. 10 Processing or formulation factors that can modify the effect of high pressure (1) Temperature: – High temperatures increase the effect of high pressure against microorganisms and meat components – Sub-zero temperatures can protect fish products components, keeping reasonable microbial inactivation capacitykeeping capacity pH: – Low pH values increase the effect of high pressure against microorganisms and meat components

11. 11 Processing or formulation factors that can modify the effect of high pressure (2) Bacteriocins: – Some bacteriocins, and specially nisin, are very effective combined with high pressure, even on Gram – bacteria Water activity: – With higher water activity, more effectiveness of high pressure processing, but also more recovery from sub lethally injured microorganisms

12. 12 Processing or formulation factors that can modify the effect of high pressure (3) Preservatives: – Lactate addition reduce the initial inactivation of the microorganisms but it delays its later recovery Combination with other technologies: – Combined processes can improve high pressure effects on microorganisms

13. 13 Benefits of high pressure Adapted to sanitize products where heat processing is inappropriate (dry cured ham, fermented meat products, sliced ready to eat meat products, etc.) Alternative to heat treatments to process foods, inactivating micro organisms without changing the sensory qualities or the nutritional values of foods

14. 14 Other advantages of high pressure Very low use of energy No residues: uses only tap water Safe for workers Accepted by consumers and retailers Does not produce – new chemical compounds – radiolytic by-products

15. 15 Present range of pressures available

16. 16 Processing temperatures

17. 17 Economic data MODELProductivity (a)Cost (b) Wave 6000/55170 kg/h0,19 € / kg Wave 6000/150425 kg/h0,14 € / kg Wave 6000/300850 kg/h0,11 € / kg Wave 6000/4202 000 kg/h0,05 € / kg (a) Filled at 50% volume and processing time 3 minutes at 600 MPa (b) Calculated for 5 years depreciation, production 280 days/year, 16 h/day

18. 18 Industrial motivations to use High Hydrostatic Pressure treatment Higher microbiological safety – Significant reduction of the risks associated with the presence and growth of pathogens – Additional preservation treatment applied to the products after packaging Better sensorial quality – Significant reduction of the sensorial risks associated to the presence and growth of alteration microorganisms (fresher flavour)

19. 19 Reasons for the industrial use of HHP Consumer satisfaction – Fresher flavor – Healthy or convenient attributes Legal requirements – Related to food safety – Related to export regulations As a reaction in front a food crisis To meet costumers’ specifications Shelf life extension Image of innovative company – Both to retailers and end users – Producing safer and reliable products Technology fully accepted by consumers

20. 20 Examples of uses of HHP Dry-cured meat:

21. 21 Examples of uses of HHP Cooked meat: cooked ham, roasted chicken, turkey breast, cooked sausages

22. 22 Examples of uses of HHP Ready meals:

23. 23 Obstacles for the implementation of HHP (1) Low level of public knowledge about the technology Attitude of the public administrations in Europe – Legal risk perception: no explicit authorization in most cases – Lack of an explicit support for a wider use of the technology Image of high cost – Real costs are lower than perceived costs – Need of processing centers providing HPP contract processing to SME’s False image of high technological sophistication

24. 24 Obstacles for the implementation of HHP (2) Reduced number of suppliers for equipments – More options available would encourage a more active market Perception of economical risk – Doubts about mechanical and structural reliability (unjustified) – Some fear about the usual quick obsolescence for the first versions of industrial equipments – Initial high investment cost (mainly for big companies with need for simultaneous purchasing of multiple equipments) – It explains why the average user of high pressure is a medium size company managed by the owners – It’s necessary to offer to food companies approved protocols and process validation services for different food matrixes