1. Tissue Engineering Lecture 19, 4/16/15 Paper Review Cell Origami

2. What is the motivation of this study? Origami, the traditional Japanese art of paper folding, has remained popular over the centuries because it enables the production of various three-dimensional (3D) sculptures simply by folding two-dimensional (2D) sheets. This paper describes a method of generating three- dimensional (3D) cell-laden microstructures by applying the principle of origami folding technique and cell traction force (CTF) Cells naturally exert a contractile force [24], known as the cell traction force (CTF), that is generated by actomyosin interactions and actin polymerization, and pulls toward the center of the cell body (Figure 1A).

3. Figure 1a-c: Jemima Lamothe

4. Figure 1d-g: Robert Gaspardi MPC

6. Figure 2: Thanh Nguyen

7. Figure 3: Michael Grunwald

8. Movies! Movie S1

9. No flexible joint A: Cells seeded on to microplate exhibit maximum angle when cells contact B: Before and after phase contrast images of microplates Angle depends directly on number of cells and not microplate thickness Scale bar: 50 μm Figure 4a-b: Cody Siroka

10. Figure 4c-e: Brittany Shepler

11. Movies! Movie S2

12. Figure 5: Eric Stowe

13. Movies! Movies S3-5

14. Figure 6: Kyle Pariseau

15. Movies! Movie S6

16. Conclusions, Perspectives Authors used cell traction to drive the folding of 2D sheets into 3D cell-laden microstructures (Cell origami) They could make cubes, soccer balls, and tubes. They propose this technique could be applied to make devices such as stents/grafts, and as new 3D environments in which to study cells.

17. Next week! Last paper is next week. I am swapping it out!! Don’t read it yet!! I’ll email out the paper and modified figure assignments ASAP. First presentations are next Thursday!!