1. Next Generation 2100 Software - A Wealth of New Features

2. Slide 2Chairperson: Ingrid Ginnutt Content of the E-seminar 2100 expert software concept New features How to use new features? New product structure Your upgrade to new 2100 expert SW Summary

3. Slide 3Chairperson: Ingrid Ginnutt The Agilent 2100 Bioanalyzer DNA 7500 LabChip kit Sizing and Quantitation (100-7500bp) Restriction digests, PCR products DNA 12000 LabChip kit Sizing and Quantitation (100-12000bp) Restriction digests, larger DNA fragments RNA 6000 Nano LabChip kit QC & Quantitation (up to 6000 bases) Total RNA, mRNA, microarray samples DNA 1000 LabChip kit Sizing and Quantitation (25-1000bp) Small PCR products, small restriction digests Protein 200 Plus LabChip kit Sizing and Analysis (14-200 kDa) Proteins DNA 500 LabChip kit Sizing and Quantitation (25-500bp) PCR products, restriction digests Cell Fluorescence LabChip kit Analysis of cell fluorescence parameters Cells RNA 6000 Pico LabChip kit High Sensitivity QC (up to 6000 bases) Total RNA, mRNA Protein 50 LabChip kit Sizing and Analysis (5-50 kDa) Proteins

4. Slide 4Chairperson: Ingrid Ginnutt Winter 2004Dec 2003 2100 expert software - one version for all assays - Decl. of system validation 2100 expert security pack - 21 CFR part 11compliance - electronic records - electronic signatures - audit trails 2100 bioanalyzer - IQ and OQ/PV services - Declaration of conformity Chips and reagents - Declaration of conformity SW for Molecular Assays: “Bio sizing” SW for Cell Assays: “Cell fluorescence” HW and kits Software 2100 Software Roadmap * Biosizing and Cell fluorescence will be made obsolete * * Past

5. Slide 5Chairperson: Ingrid Ginnutt 2100 Expert Software – In Brief Replaces Biosizing and Cell fluorescence software One SW for electrophoresis and flow cytometry assays supporting the platform idea: “one platform – endless possibilities” Incl. all capabilities of the current “Biosizing” and “Cell fluorescence” SW One look and feel for the analysis of RNA, DNA, proteins and cells Support all existing and future LabChip kits and assays Import of old data files from Biosizing and Cell Fluorescence possible

6. Slide 6Chairperson: Ingrid Ginnutt 2100 Expert Software – In Brief (cont.) Control of all bioanalyzers (old A- & B- and current C-versions) Data Organizer not compatible with 2100 expert License keys for software modules (electrophoresis, flow cytometry, security pack), no license for data review Runs on Windows 2000 and Windows XP Many new SW features

7. Slide 7Chairperson: Ingrid Ginnutt 2100 Expert Requirements PC hardware (minimum requirements): CPU: Pentium III with 800 MHz RAM: 128 Mbyte Ports: 1-2 serial ports VGA: Resolution of 1024 x 768 HDD: 10 GB Operating system: Windows 2000 Service Pack 4 (or higher) Windows XP Service Pack 1 (or higher) Not compatible with Windows NT 2100 bioanalyzer: A and B/C version, compliance services for B/C version only (serial number DE13701001 or higher)

8. Slide 8Chairperson: Ingrid Ginnutt 2100 Expert New Features - Based on User Requirements Improved integrator with manual integration (change start/end point and baseline) Color coded result flagging (e.g. “mark all samples in green that contain a 38 kDa protein in a concentration above 75 ng/ul”) Compare results from different chips within the improved and integrated data comparison context (previously Data Evaluation Tool) Pattern recognition and smear analysis applicable to RNA, DNA and protein assays Normal and advanced user mode Improved reporting and printing functions Preview in file open window (e.g. gel-like image, sample names)

9. Slide 9Chairperson: Ingrid Ginnutt 2100 Expert New Features - Based on User Requirements (cont.) Customizable result tables and gel like-image More export formats (txt, pdf, wmf, jpg, tif, bmp,...) New context structure for easy access of software functionalities Extended diagnosis functionality Support of IQ & OQ/PV Prerequisite for 21 CFR part 11 compliance, prepared for security pack (with user authentication/user roles, archiving/revision control, audit trails, …..) And many more ……

10. Slide 10Chairperson: Ingrid Ginnutt 2100 Expert Contexts Instrument context Connect to up to 2 instruments Run a demo assay Run an assay Data and Assay context Enter sample info Review results (gel-like image, E-gram, dot plot, histogram, result table) Change assay set points on sample or chip level (e.g. Min. Peak Height) Result flagging Create/modify new assays Validation context Run IQ and OQ for hardware and software Comparison context Compare results from different chips (gel-like image, E-gram, result table)

11. Slide 11Chairperson: Ingrid Ginnutt Tree view for navigation between samples and files Context menu bar Customizable gel-like image (change order) Customizable result table (change order and add additional columns) Single gel lane for selected E-gram Task bar with context sensitive icons for different actions Tabs for different data analysis functions

12. Slide 12Chairperson: Ingrid Ginnutt How to use old data files from Biosizing or Cell Fluorescence? Go to File/Import Select old bioanalyzer files that you would like to import Note: 2100 expert files can not be imported in Biosizing or Cell Fluorescence

13. Slide 13Chairperson: Ingrid Ginnutt Convenient switching between flow cytometric and electrophoretic assays Flow cytometric and electrophoretic assays are available in the Assay menu depending on the inserted cartridge and the entered license key(s)

14. Slide 14Chairperson: Ingrid Ginnutt Click on Electropherogram tab Switch to Manual Integration mode select Menu/Manual integration or clicking on the “manual” icon in the task bar Easy access to manual integration in electropherogram tab

15. Slide 15Chairperson: Ingrid Ginnutt Integration of Soy Bean Protein - Get better quantitation precision with manual integration Biosizing 1 2 3 4 Conc. [ug/ml] Peak 24.834.738.029.7 4321 2100 expert - automatic integration 1 2 3 4 Conc. [ug/ml] Peak 24.834.637.929.7 4321 1 2 3 4 Conc. [ug/ml] Peak 28.638.044.433.6 4321 2100 expert - manual integration 1 Conc. [ug/ml] Peak 144.6 1

16. Slide 16Chairperson: Ingrid Ginnutt How to move peak start/end point in manual integration mode? Peak start and end time are shown as blue dots (A) blue dot is on signal : Click on blue dot Dot turns green Hold left mouse button and move dot along signal (B) blue dot is on drop line (dotted line) Click on blue dot Dot turns green Press Ctrl and hold left mouse button to move dot along drop line Create a new drop line Press Ctrl and hold left mouse button to move dot along new drop line (A) (B)

17. Slide 17Chairperson: Ingrid Ginnutt How to modify columns displayed in result table? Right mouse click on table headers Select Configure columns Select to be displayed column headers

18. Slide 18Chairperson: Ingrid Ginnutt How to change the column order in the result table? Click on column header and move it to desired place New location is indicated by green arrow

19. Slide 19Chairperson: Ingrid Ginnutt How to apply set points from one data file to another? Go to Assay Properties tab in the data context Click on Import set points Select the file from which you want to apply the set points from

20. Slide 20Chairperson: Ingrid Ginnutt How to get a quick result overview using color- coded result flagging? Set up rules in Result flagging (data and assay context) Apply rules DNA fragment with 25, 70, 93 bp found DNA fragment with 160, 206, 308 bp found DNA fragment with 500, 610, 1000 bp found and total conc. > 50 ng/ul

21. Slide 21Chairperson: Ingrid Ginnutt How to re-arrange the gel-like image lanes for better comparison? Select the lane that you would like to move Hold left mouse click on lane Move lane to new location Initial gel-like image Rearranged gel-like image

22. Slide 22Chairperson: Ingrid Ginnutt How to zoom in the gel-like image? Hold left mouse click on gel-like image Two horizontal lines appear (one line is fixed) Move mouse while holding left button Release left button (second line is fixed Both line define the zooming window Double-click to undo the zoom

23. Slide 23Chairperson: Ingrid Ginnutt How to do smear analysis for RNA, DNA or protein assays? Go to Assay properties Select Smear analysis in the advanced sample set points Define size regions Results are displayed in new Region table Regions are indicated by dotted lines in the electropherogram

24. Slide 24Chairperson: Ingrid Ginnutt Compare chips in comparison context Select sample from different chips Electropherogram overlay Legend for electropherogram overlay Single gel lane for selected E-gram Save selected samples in a comparison file Switch between comparison summary, gel and electropherogram view

25. Slide 25Chairperson: Ingrid Ginnutt New Product Structure - Start with flow cytometry or electrophoresis functionality or both ! 2100, Accs. 2100 G2938C 2100, PC, Accs., VM SW on CD Start-up service 2100 Desktop System G2940CA 2100, PC, Accs., VM SW on CD Start-up service 2100 Laptop System G2943CA Electrophoresis Set G2947CA Electrode Cartridge Electroph. License Flow Cytometry Set G2948CA Pressure Cartridge Check.&Test chip kit Flow Cyt. License System IQ and OQ/PV Services: Per incident: IQ (R1015A) OQ/PV (R1016A)

26. Slide 26Chairperson: Ingrid Ginnutt Your upgrade to 2100 expert software Free upgrade of current 2100 users, if registered after 2100 installation with LabChip kit fax back form Software and electrophoresis licenses are in the post or have been shipped already if you have not receive your copy by March 2004, please contact your local sales representative current cell fluorescence users please contact your local sales representative to acquire a flow cytometry license 2100 expert software can be run in parallel to Biosizing or Cell fluorescence software

27. Slide 27Chairperson: Ingrid Ginnutt Compliance for the Agilent 2100 bioanalyzer systems - A 2-phased approach Phase I – GMP/GLP compliance: 2100 expert software IQ & OQ/PV services "Declarations of Conformity" for all LabChip kits Phase II – full compliance: Security Pack for full 21 CFR Part 11 compliance

28. Slide 28Chairperson: Ingrid Ginnutt Reasons to switch to 2100 expert software Nothing to loose – all features from Biosizing and Cell fluoresence included Benefit from many new software features Take first step towards full 21 CFR part 11 compliance New assays and LabChip kits will only be developed for 2100 expert “One software, one platform for analysis of cells, proteins, RNA and DNA”