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5. RF-C is a five-subunit complex All subunits are related in sequence and have ATP binding motifs ATP hydrolysis by RF-C is associated with the loading of PCNA RF-C is the functional homolog of the clamp-loader  complex

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7. Polymerase switching occurs even on lagging strands; pol  does most of DNA synthesis

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9. How do you think the PCNA is removed after ligation?

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11. PCNA interacts with RF-C, pol , Fen1, DNA ligase, CAF1 and MCMT Several of these have a common motif used in the interaction: Q-X-X-L/I/M-X-X-F- F/Y p21/CIP1/WAF1, a protein induced by the tumor suppressor p53 uses the same motif to interact with PCNA What effect is p21 expected to have on DNA replication/repair?

12. 免疫学信息网 http://www.immuneweb.com FEN1 interacts with PCNA

13. 免疫学信息网 http://www.immuneweb.com DNA Helicase * Helicase binds ss DNA * Hydrolyzes ATP to move along ssDNA and peel of substrate DNA * Can move 5 ‘ to 3 ’ or 3 ‘ to 5 ’ or in both directions on ssDNA and is classified as such * Dimers or hexamers

14. 免疫学信息网 http://www.immuneweb.com Conserved helicase motifs: Includes the following for ATP binding and hydrolysis I = Walker A motif GxGxGKT II = Walker B motif  DEad Helicases are ATP driven molecular motors