1. DO NOW:
Take out your microscope YELLOW packets from last week.
In your NOTES section of your binder, please list EIGHT differences between these two pictures.

3. Stare at the red dot on the girl’s nose for 30 seconds.
Turn your eyes to a plain surface.
Blink repeatedly and quickly.

9. Using a Compound Light Microscope Directed Reading

10. 1.) The magnifies the image x. 2.) The low- magnifies the image x.
Using the Microscope
1.) The magnifies the image x.
2.) The low magnifies the image x.
3.) The high magnifies the image either x or x.
ocular lens 10
power objective 10
power objective 43 40

11. Using the Microscope
4.) The holds the objective and can be turned to change from one to the other.
5.) The maintains the correct distance between the and .
6.) The moves the body tube and down to allow focusing of the
revolving nosepiece
magnification
body tube
ocular lens
objectives
coarse - adjustment knob
image up

12. 7.) The moves the body tube to bring the image into focus.
Using the Microscope
fine- adjustment knob
7.) The moves the body tube to bring the image into focus.
8.) The supports a slide.
9.) hold the slide in place for .
slightly
sharper
stage
Stage clips
viewing

13. 10.) The controls that amount of coming through the stage.
Using the Microscope
10.) The controls that amount of coming through the stage.
11.) The light sources provides a for viewing the .
12.) The supports the body .
diaphragm
light
light
slide
arm
tube

14. Using the Microscope
13.) The supports the . 14.) Inclination joint (hinge): Allows the upper part of the microscope to be tilted back to view the specimen while seated.
base
microscope

15. Proper Use of the Compound Light Microscope
1.) How would you carry a microscope?
With your left hand
With your right hand
With both hands

16. Proper Use of the Compound Light Microscope
2.) How many centimeters should the microscope be from the edge of a table?
1 cm
5 cm
10 cm

17. Proper Use of the Compound Light Microscope
3.)  What power objective should you ALWAYS begin working with?
Low
Medium
High

18. Proper Use of the Compound Light Microscope
4.)  What must be adjusted to get the microscope into low power?
Revolving objective
Revolving nosepiece
Revolving ocular lens

19. Proper Use of the Compound Light Microscope
5.) Once you place a prepared slide over the hole in the stage, what secures the slide?
A rubber band
Your finger
Stage clips

20. Proper Use of the Compound Light Microscope
6.) If the diaphragm is moved, what gets adjusted?
The height of the stage
The amount of light
The objective lens

21. Proper Use of the Compound Light Microscope
7.) Once you are looking at the stage from eye level, you should slowly turn the:
Fine adjustment knob
Medium adjustment knob
Course adjustment knob

22. Proper Use of the Compound Light Microscope
8.) You should NEVER allow the objective to touch the slide
True
False
Sometimes it can

23. Proper Use of the Compound Light Microscope
9.) While looking through the ocular lens, you should turn the coarse adjustment knob to raise the low-power objective so that the image is:
Focused
Blurred
Not visible

24. Proper Use of the Compound Light Microscope
10.) Once the object is focused, what sharpens the image?
Fine adjustment knob
Medium adjustment knob
Coarse adjustment knob

25. Proper Use of the Compound Light Microscope
11.)  When you are in high power you should always use the coarse adjustment knob?
True
False
Sometimes

26. Proper Use of the Compound Light Microscope
12.) When you are finished using the microscope, you should remove the slide and clean the ocular and objective lens with what type of paper?
Lens paper
Sand paper
Construction paper

27. Proper Use of the Compound Light Microscope
13.) When you are all finished using the microscope, you should always:
Return it to the storage area
Leave it where it is
Throw it away

28. Making a Wet Mount Slide
1.) Use sand paper to clean a glass slide and a coverslip.
LENS

29. Making a Wet Mount Slide
2.)  Place the specimen you wish to observe on the left of the slide.
CENTER

30. Making a Wet Mount Slide
3.)  Using a medicine dropper, place 5 drops of water on the specimen.
ONE

31. Making a Wet Mount Slide
4.)  Hold the coverslip at the edge of the water at a 90 degree angle to the slide. Make sure the water runs along the middle of the coverslip.
45 & EDGE

32. Making a Wet Mount Slide
5.)  Lower the coverslip quickly to avoid trapping air bubbles.
SLOWLY

33. Making a Wet Mount Slide
6.)  Water might evaporate from the slide as you work. Remove more water to keep the specimen fresh.
ADD

34. Making a Wet Mount Slide
6a.)  Place the tip of the medicine dropper next to edge of the coverslip. Add 4 drops of water.
A (ONE)

35. Making a Wet Mount Slide
6b.)  Remove excess water from the slide by using the center of a paper towel as a blotter.
CORNER

36. Making a Wet Mount Slide
6c.)  Lift the coverslip to add or remove water.
DO NOT