1. Unit 8 test
Biotech study guide

2. Test format 20 questions 1 open-ended (10 points) Know vocabulary
Be able to place the steps for recombinant DNA technology
1 open-ended (10 points)
Be able to read a DNA fingerprint
Explain the process of gel electrophoresis
How this technology is used to solve crimes

3. What kind of cells are used to make animal clones?

4. What kind of cells are used to make animal clones
What kind of cells are used to make animal clones? Body cells and egg cells

5. This is a unique gel electrophoresis banding pattern produced by a person’s restriction fragments

6. This is a unique gel electrophoresis banding pattern produced by a person’s restriction fragments DNA fingerprint

7. Similar to DNA replication because it makes lots of copies of the DNA molecule

8. Similar to DNA replication because it makes lots of copies of the DNA molecule PCR

9. Small circular DNA molecules that are naturally found in bacteria

10. Small circular DNA molecules that are naturally found in bacteria plasmids

11. Knowing the sequence of an organism’s DNA allows scientists to study…

12. Knowing the sequence of an organism’s DNA allows scientists to study… specific genes

13. This is combining genes from different sources into a single DNA molecule

14. This is combining genes from different sources into a single DNA molecule recombinant DNA

15. What process separates DNA fragments by their size and charge

16. What process separates DNA fragments by their size and charge gel electrophoresis

17. During genetic modification, the organism’s cells take in ________ from another ___________.

18. During genetic modification, the organism’s cells take in __DNA____ from another __source____.

19. What was added to the blood samples to create these fragments?

20. What was added to the blood samples to create these fragments?
Restriction enzymes

21. What is this process?

22. What is this process? Gel electrophoresis – be able to explain this process

23. Who committed the crime?

24. Who committed the crime? Suspect 2

25. What would be possible sources for these DNA samples?

26. What would be possible sources for these DNA samples
What would be possible sources for these DNA samples? Blood, hair, skin cells, bodily fluids

27. What is the correct order?
____ Insert recombinant DNA into bacteria cells
____ Isolate desired product from bacteria cells
____ Cut plasmid and desired gene with the same restriction enyzmes
____ Clone bacteria cells containing recombinant DNA
____ Combine plasmid and gene fragment, creating recombinant DNA

28. What is the correct order?
__3_ Insert recombinant DNA into bacteria cells
__5_ Isolate desired product from bacteria cells
__1_ Cut plasmid and desired gene with the same restriction enyzmes
__4_ Clone bacteria cells containing recombinant DNA
__2_ Combine plasmid and gene fragment, creating recombinant DNA

29. What was used to cut this piece of DNA?

30. What was used to cut this piece of DNA? Restriction enzymes

31. What are C & D?

32. What are C & D? sticky ends

33. If a scientists wants to make a human protein (like insulin) using bacteria cells – what would be their first step?

34. If a scientists wants to make a human protein (like insulin) using bacteria cells – what would be their first step? Use a restriction enzyme to cut out the human gene and the plasmid

35. A stretch of DNA that varies among individuals is called a…

36. A stretch of DNA that varies among individuals is called a… genetic marker

37. In this gel sample, band 3 moved further than all the other bands. Why?

38. In this gel sample, band 3 moved further than all the other bands. Why
In this gel sample, band 3 moved further than all the other bands. Why? Moved faster because it is smaller than other pieces

39. What are some advantages to transgenic (GMO) plants?

40. What are some advantages to transgenic (GMO) plants
What are some advantages to transgenic (GMO) plants? More nutrients resist pests resist herbicides longer shelf-life