1. A 3-week Biotech Unit for Any Biology Class Ellyn Daugherty SMBCP, San Mateo CA Educator/Author www.BiotechEd.com www.SMBiotech.com www.emcp.com/biotech www.sargentwelch.com/biotech

2. Biotech in Biology 3 weeks (15 hours) > What is Biotechnology? Computer Search Collage > Micropipeting Activity > DNA Spooling Activity > DNA Fingerprinting Using RFLPs Activity > Recombinant DNA Technology > Bacterial Transformation (GMO) Activity > Bioethics of Engineering Organisms > Careers in Biotechnology

3. Materials, Supplies, Funds Needed for Biotech in Biology Unit Class set of: 4 micropipets/tips each (P-1000, P-200, P-20) 4 gel boxes, 2 power supplies DNA Spooling Activity Kit DNA Fingerprinting Activity Kit Bacteria Transformation Activity Kit Other supplies and materials (96-well plates, food coloring, 37°C incubation oven, water bath = about $300 in kits/ class = about $3000 for school set of micropipets and gel apparatus

4. What Is Biotechnology?

5. Micropipeting Activity Show me: Pipet 135 µL or 235 µL into a tube

6. What Does DNA Look Like? (Edvotek #S-10) = one 50 min class period (30 min activity, 20 min discussion $39 / 10 lab groups DNA Spooling Activity Do Onions Have DNA (or other fruits or veggies) ? (Edvotek #S-75) = one 50 min class period (30 min activity, 20 min discussion $49 / 5 lab groups … or use my Lab 4b Materials from SW List

7. DNA Fingerprinting Using RFLPs Activity Ward’s DNA Detectives Lab Activity #109) = four 50 min class periods $115 / 8 lab groups Edvotek’s DNA Fingerprinting by Restriction Fragmentation Patterns #109) = four 50 min class periods $79 / 6 lab groups

8. Recombinant DNA Technology use http://www.dnalc.org/resources/animations/ Use the Restriction Digestion animation to discuss: Restriction enzymes DNA ligase Recombinant DNA (rDNA) Use the Transformation animation to discuss: Insertion of rDNA vector Selection and Expression of newly insert genes Applications of rDNA technology

9. Bacterial Transformation (GMO) Activity Ward’s Improved Bacterial Transformation Using GFP #36 V 6359 = four 50 min class periods $85/ 8 lab groups Edvotek’s Transformation with Green Fluorescent Protein #223 and #969 = four 50 min class periods $110/ 10 lab groups

10. Biotechnology Career Research e-Project

11. Bioethics of Engineered Organisms

12. Materials Lists www.sargentwelch.com/biotech Find your Sales Rep for materials/equipment/ support

13. Biotechnology: Science for the New Millennium Ellyn Daugherty ellyn@BiotechEd.com Text with Encore CD Lab Manual Instructor’s Guide Course Planner (Lesson Plans) Instructor Resource Center on web Student Notebook, Test Generator, etc. available www.BiotechEd.com for tour through the entire curriculum, ancillaries, websites, and professional development opportunities