1. Forensics Chapter 8

2. Central Points  DNA testing can determine identity  DNA profiles are constructed in specialized laboratories  DNA profiles used in courts  Ancestry can be determined by testing DNA  May refuse to give sample to the police

3. 8.1 How Is DNA Tested?  In 1975, in England  Dr. Alec Jeffreys developed DNA fingerprinting to compare DNA profiles from different individuals  Used to solve murders (see Narborough Village Murders)

4. Spotlight on Law: Narborough Village  In 1989, first case that used DNA profile for evidence  Rape and murder of two girls in a small English village within 3-year period  Buckland confessed to one murder  DNA analysis, one man killed both but not Buckland  DNA dragnet of > 4,000 men, no match  Colin Pitchfork paid someone to give sample but caught because of overheard conversation

5. 8.2 DNA Profile (DNA Fingerprint)  Variations in: Minisatellites (repeated sequences, 10–100 base pairs) Short tandem repeats (STRs) (2–9 base pairs) Single nucleotide polymorphisms (SNPs)  DNA variations can be used to create DNA profile and ID individuals  Used in criminal cases, paternity lawsuits, and studies of human evolution

6.  Minisatellites  Short tandem repeats (STRs)  Single nucleotide polymorphisms (SNPs) Variations in DNA...CCTGACTTAGGATTGCCA...

7. Methods to Prepare DNA Profiles Two common methods:  Restriction fragment length polymorphism (RFLP) analysis  Polymerase chain reaction (PCR)

8. RFLP Analysis  Large amount of blood or tissue Steps: 1.DNA extracted 2.Cut into fragments using restriction enzymes 3.Fragments loaded on agarose gel 4.Electrical current passed through gel, DNA fragments migrate and separating by size 5.Pattern fragments photographed and compared to others

9. Steps in RFLP

10. RFLP

11. PCR  Small amount of DNA sample Steps in one cycle: 1.DNA placed in solution 2.Heated, double-stranded DNA separates 3.Temperature lowered, primers mixed with DNA 4.Primers pair with complementary regions on DNA 5.DNA polymerase uses nucleotides to synthesize double-stranded DNA molecule

12. Steps in PCR

13. Using PCR to Increase DNA

14. Animation  http://www.dnalc.org/resources/3d/19- polymerase-chain-reaction.html http://www.dnalc.org/resources/3d/19- polymerase-chain-reaction.html

15. STR: Allele, a Unique Number of Repeats  2–9 nucleotides long  Example of three alleles: Allele 1: …AGA… Allele 2: …AGAAGAAGA… Allele 3: …AGAAGAAGAAGAAGA…  Analysis of several STR alleles show frequency of specific combinations

16. PCR for DNA Profile Steps: 1.DNA sample analyzed for STR alleles present in population members 2.Analyze population frequency, how often combinations of alleles present 3.Population frequencies for each STR allele multiplied to estimate probability

17. Combined Frequencies

18. DNA Samples (1)

19. DNA Samples (2)

20. Animation: Studying and Manipulating Genomes (DNA fingerprinting)

21. Animation: Whodunnit? A DNA fingerprint crime

22. 8.3 DNA Profiling and U.S. Courts  Landmark case: Frye v. U.S.  DNA entered U.S. court system in 1989  Approved for use in courts all over the country  Evidence may be challenged

23. How Are DNA Profiles Used?  Material from crime scene analyzed, compared to other samples or data base  Combined DNA Index System (CODIS) panel of 13 STRs for DNA profiles  ~30% of DNA profile results clear innocent people

24. DNA Profile Comparison

25. DNA Databases  Since 1998, FBI enters DNA profile from felons  More than 1,700,000 profiles  DNA databases becoming important tools in solving crimes

26. 8.4 Other Uses for DNA Profiles  Czar Nicholas II and family  Remains of military personnel killed in action  Victims of 9/11 and Katrina  Paternity identification  Trace our ancestry

27. Czar Nicholas II

28. Tracing Ancestry  Mitochondrial DNA testing Mitochondria organelle with DNA Maternal inheritance, passed from mother to children Use haplotypes to trace maternal ancestry  Y chromosome testing Y chromosome passed father to son Use haplotypes to trace paternal inheritance

29. Mitochondrial Inheritance

30. Y Chromosome Inheritance