2. POLYMERASE CHAIN REACTION AMPLIFYING DNA What do you need to replicate DNA? http://www.youtube.com/watch?v=uD umZT5z5R8

3. http://www.dnalc.org/view/15475-The-cycles-of-the- polymerase-chain-reaction-PCR-3D-animation-with-no- audio.html

4. WHICH DNA SEQUENCE IS AMPLIFIED?

6. TANDEM REPEATS (VNTR)

7. TANDEM REPEAT GENES 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’

8. DETERMINING YOUR ALLELES CREATING DNA FINGERPRINT USING GEL ELECTROPHORESIS

9. SAMPLE GEL Ladder Suspect 1 Suspect 2 Crime Scene 5’TTAACCTTAACCTTAACCTTAACCTTAACCTTAACC3’ 5’TTAACCTTAACCTTAACC 3’

10. HOW UNIQUE ARE YOU?

11. RFLP RESTRICTION FRAGMENT LENGTH POLYMORPHISM

12. EXAMPLE USE TTCC RESTRICTION ENZYME DNA SAMPLE ONE: ACTGTTCCACACTTCCGCACATCTTAATTCC DNA SAMLE TWO: GTTCCATGCCGAGTACGTTCCATGCTA

13. I AM YOUR FATHER….WELL MAYBE