1. Unit 8 Vocabulary 1. micropipette- tool used to transfer small volumes of liquid 2. P 20 micropipette- transfers 2µL - 20µL of liquid; the pipette we used for our lab 3. P 200 micropipette- transfers 20µL – 200 µL of liquid

2. Unit 8 Vocabulary 4. microliter (µL)- one millionth of a liter 5. milliliter (mL)- one thousandth of a liter

3. Unit 8 Vocabulary 6.microcentrifuge- machine used to spin small volumes of liquids at high speeds; must be properly balanced

4. Unit 8 Vocabulary 7. microtube- a small plastic tube used in biotechnology that holds up to 1.5 - 2 mL 8. blank- a tube containing water used that is used to balance a microcentrifuge as needed

5. Unit 8 Vocabulary 9. DNA fingerprinting- using enzymes to cut up the DNA for one or more genes in a pattern that can be matched to other samples in paternity and criminal cases Crime: Paternity:

6. Unit 8 Vocabulary 10. biotechnology- using biological techniques for practical applications, such as genetically modified foods 11. bioethics- the study of the moral concerns associated with biological research and applications

7. Unit 8 Vocabulary 12.electrolysis- the breaking of H 2 O into H + and OH - using electricity 13. electrophoresis- using an electrical current to separate DNA fragments according to length

8. Unit 8 Vocabulary 14.gel box- holds buffer and gel tray; has lid to protect user from electric current

9. Unit 8 Vocabulary 15. agarose gel- Jell-O-like substance poured into gel tray; acts as a filter for DNA

10. Unit 8 Vocabulary 16.What is the charge of DNA: positive, negative, or neutral? Negative 17. Why does the charge of DNA matter when running a gel? Since DNA is negative, it is repelled by the negative charge of the electrons flowing in the electricity, causing the DNA to move

11. Unit 8 Vocabulary 18.allele- a version of a gene 19.Heterozygous- 2 different alleles for a gene 20. Homozygous- a pair of the same allele for a gene

12. Unit 8 Vocabulary 21.restriction enzyme- cut DNA at certain sequences; used in DNA fingerprinting; ex- HinDIII

13. Unit 8 Vocabulary 21.buffer- prevents large changes in pH 22. loading dye- helps DNA to sink into well; helps you to track where DNA sample is

14. Unit 8 Vocabulary 24. H 2 O- water25. paternity- the state of being a father

15. Unit 8 Vocabulary 26. M 0 - mother’s DNA, uncut 27. M + - mother’s DNA, cut 28. B 0 - baby’s DNA, uncut 29. B + - baby’s DNA, cut 30. S 0 - suspect’s DNA, uncut 31. S + - suspect’s DNA, cut