1. Patterns of gene expression Drosophila embryogenesis in

2. RNA insitu and imaging pipeline Richard Weiszmann 96-well plate RNA insitu DGC1 and DGC2 Elaine Kwan production snapshots Amy Beaton imaging and annotation

4. Status report - to date we captured 25000 high resolution images - we examined approximately 2500 genes - images were captured for over 1200 genes

5. Correlation of microarray and image data

6. Novel insitu pattern – is it real ?

7. Annotation Collaboration with Volker Hartenstein

8. differentiated organ malpighian tubules tracheal system oenocytes fat body

9. anlage in statu nascendi dorsal ectodermmesoderm procephalic ectoderm hindgut

10. primordium malpighian tubules posterior midgut anlage head mesoderm large intestine

11. anlage in statu nascendi anlageprimordiumdifferentiated organ ~~~ stage 4-6 mesectoderm anlage in statu nascendi ~ stage 7-8 mesectoderm anlage ~ stage 9-12 midline primordium ~ Tracing back single-minded stage 13-16 midline glia

12. - allows integration with existing gene expression data - annotation terms can be easily expanded for greater detail Gene ontology -Flybase controlled vocabulary for anatomy (collaboration with Michael Ashburner). - allows sophisticated searches of the dataset

13. http://www.fruitfly.orgComing soon to collaboration with Suzanna Lewis and Shu ShengQiang

14. Three adjacent Brachyury/T-Box genes at 66E4

15. CG7312 - earmuff (encodes a Zinc_Finger_C2H2, EF_hand protein) Drosophila ZebrafishXenopus Yang, Z. et.al. Matsuo-Takasaki, M. et.al.

16. Acknowledgements Sue Celniker Richard Weiszmann Amy Beaton Elaine Kwan Volker Hartenstein Michael Ashburner Suzanna Lewis Shu ShengQiang Gerry Rubin