1. Exercise I: Isolation Analysis Plasmid DNA
Topic 8: DNA
Exercise I: Isolation Analysis Plasmid DNA
Topic 7: Cellular Genetics.
Exercise II Mitosis Cell Cycle
Exp: Cell cycle onion tips

2. Structure of Escherichia coli.

3. Two strains of E.coli: pBR322 and pUC18.

4. Purification Plasmid DNA 1:

5. [Note on pipetting:]

6. Purification Plasmid DNA 1:

7. Purification Plasmid DNA 1:

8. Spin and remove most contaminants:

9. Purification Plasmid DNA 2:

10. [Addition of very small volumes:]

11. DNAse and RNAse treatment:

12. Preparing the Agarose gel:

13. Agarose Gel.

14. Electrophoresis:

15. Electrophoresis:

16. Electrophoretic Mobility of Nucleic Acids:

17. pBR322
pUC18
ET D R
ET D R

18. pUC18
pBR322
ET D R
ET R D

19. MITOSIS: Onion root tip.

22. Onion root tip.

23. Onion root tip.