1. Progress Report – Week 9 Section AC, Group 2 Presented by Andrew Amodei Teammates: David Bergsman, Michael Huang, Elizabeth Lam May 24 th, 2012

2. Goals of the Week Finish individual design projects – Water for injection system – Facility layout – Downstream columns – Equipment and labor scheduling Finish debugging spreadsheet FEMA analysis Update stream tables and PFD Complete and submit Phase II report

3. Gantt Chart

4. Team progress Finished debugging spreadsheet Updated stream tables Updated PFD Finalizing individual design projects Finalizing FEMA analysis

5. Facility layout Color-coded rooms Clean steam, WFI, air, O 2, CO 2 lines Waste lines and neutralization tanks Additional holding tanks Personnel support space Sized raw material and product warehouse Added personnel and material flow arrows

6. Facility layout

7. Chromatography columns Finalized chromatography column parameters Determined pump, pipe, and valve requirements Chromatography column results CriteriaProtein AIEXHIC ResinMabSelectSepharose FF Phenyl Sepharose6 FF (high sub) Number222 Diameter [cm] 80100 Flow Rate [cm/hr] 100 Average Process Time [hr] 27.49.527.5 Pump and pipe specifications PumpPipe Type Positive Displacement Material Stainless Steel Power [hp]1Diameter [cm]13 Capacity [L/min] 45.42Unit Cost [$/1.5m]100 Unit Price [$] 8,500 Pipeline Distance [m] 769 Number14Number of Valves53

8. Scheduling Optimization of scheduling complete Results: Optimized scheduling details CriteriaValueComment Number of seed train rooms 2 Increases flexibility/reduced operating time Downstream lot size31,000 L Upstream train number 6 Labor scheduling PositionNumber of personnel Seed train room2per room Bioreactor room2per room Media production2 Downstream process2per downstream process Mixing buffer solution2 Autoclave operation2 WFI system2 Shift mananager Seed train1 Bioreactor1 Primary recovery1 Chromatography/TFF1

9. Water for injection Compiled equipment inventory Calculated equipment and operating costs Added and sized holding tanks Determined pump types and number Sized and priced deionization unit Equipment type and cost EquipmentTypeNumberCost [$] Clean steam generatorSteris 450-T197500 Deionization unitSiemens FlexTrex36130355 Holding tanksStainless steel237078 PumpsPeristaltic Hose425400 Vapor compression unitStilmas 4700-T21900000 Total:2090334

10. Water for injection

11. Major results Process design and optimization completed Individual and Phase II reports nearing completion Economic analysis over 30 year plant life: Economic analysis ParameterBasecaseDisposable Fixed Capital Investment [billion $]0.130.09 Total Operating Costs [billion $]9.18.94 NPV [billion $] 158 EACF [billion $] 8.0 Future Worth of Profit [billion $] 383

12. Future goals Redesign facility layout for optimized case Separate number of seed trains from number of bioreactors. Set up seed train to handle arbitrary volume / cell sizes. Prepare for presentation Rest

13. Questions?

14. Team Questions Any other economic factors to consider aside from HVAC costs? Does my ethics report grade affect my team’s overall score?