1. Chapter 14 Companion site for Light and Video Microscopy Author: Wayne

2. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. A video camera converts intensities into voltages.. FIGURE 14-1

3. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Linear amplification of an electrical signal that represents intensities. FIGURE 14-2

4. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Nonlinear amplification of the electrical signal that represents intensities FIGURE 14-3

5. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Examples of complex processing of the electrical signal that represents intensities. FIGURE 14-4

6. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Taking the first derivative of the electrical signal produces a pseudo-relief image. FIGURE 14-5

7. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Taking the second derivative of the electrical signal and subtracting it from the original signal sharpens the image. FIGURE 14-6

8. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier. FIGURE 14-7

9. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier configured to function as an inverting amplifier. FIGURE 14-8

10. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier configured to function as a noninverting amplifier. FIGURE 14-9

11. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An equivalent circuit. FIGURE 14-10

12. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier configured as an amplifier with unity gain. FIGURE 14-11

13. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier configured as a differentiator. FIGURE 14-12

14. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier configured as an integrator. FIGURE 14-13

15. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Operational amplifiers configured as a logarithmic converter. FIGURE 14-14

16. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Operational amplifiers used to return the absolute value of the input. FIGURE 14-15

17. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Operational amplifier configured as a low pass filter. FIGURE 14-16

18. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Operational amplifier configured as a high pass filter. FIGURE 14-17

19. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Operational amplifier configured as a band pass filter. FIGURE 14-18

20. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier configured as an adder connection. FIGURE 14-19

21. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. An operational amplifier configured as a subtractor connection. FIGURE 14-20

22. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Beating the Rayleigh criterion using the offset and gain controls of an analog image processor. FIGURE 14-21

23. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. The formation of a digital image. FIGURE 14-22

24. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Use Fourier transforms to correct for the degradation of the image produced by the optical system itself. FIGURE 14-23

25. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. The convolution filtering process. FIGURE 14-24

26. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. Using multipaly-stained beads to put in register images from multiply stained specimens. FIGURE 14-25

27. Companion site for Light and Video Microscopy. by Wayne Copyright © 2009 by Academic Press. All rights reserved. FIGURE 14-26 The distortion of reality and the reality of distortion.