1. Concept 6.6: The cytoskeleton is a network of fibers that organizes structures and activities in the cell The cytoskeleton is a network of fibers extending throughout the cytoplasm It organizes the cell’s structures and activities, anchoring many organelles It is composed of three types of molecular structures: –Microtubules –Microfilaments –Intermediate filaments Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

2. Fig. 6-20 Microtubule Microfilaments 0.25 µm

3. Roles of the Cytoskeleton: Support, Motility, and Regulation The cytoskeleton helps to support the cell and maintain its shape It interacts with motor proteins to produce motility Inside the cell, vesicles can travel along “monorails” provided by the cytoskeleton Recent evidence suggests that the cytoskeleton may help regulate biochemical activities Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

4. Fig. 6-21 Vesicle ATP Receptor for motor protein Microtubule of cytoskeleton Motor protein (ATP powered) (a) MicrotubuleVesicles (b) 0.25 µm

5. Table 6-1 10 µm Column of tubulin dimers Tubulin dimer Actin subunit  25 nm 7 nm Keratin proteins Fibrous subunit (keratins coiled together) 8–12 nm

6. Centrosomes and Centrioles In many cells, microtubules grow out from a centrosome near the nucleus The centrosome is a “microtubule- organizing center” In animal cells, the centrosome has a pair of centrioles, each with nine triplets of microtubules arranged in a ring Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

7. Fig. 6-22 Centrosome Microtubule Centrioles 0.25 µm Longitudinal section of one centriole Microtubules Cross section of the other centriole

8. Cilia and Flagella Microtubules control the beating of cilia and flagella, locomotor appendages of some cells Cilia and flagella differ in their beating patterns Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

9. Fig. 6-23 5 µm Direction of swimming (a) Motion of flagella Direction of organism’s movement Power strokeRecovery stroke (b) Motion of cilia 15 µm

10. Cytoskeleton Review What are 3 roles of the cytoskeleton? – Maintain shape, mechanical support, cell motility There are 3 main types of fibers that make up the cytoskeleton – what are they? – Microtubules, microfilaments, intermediate filaments Microtubles are hollow rods. What are four functions of microtubules? – Maintenance of cell shape, cell motility, chromosome movement during cell division, organelle movement

11. Cytoskeleton Review Microfilaments are solid. What is their function in a cell? – Movement Intermediate filaments are bigger than microfilaments but smaller than microtubules. What is their function? – Maintenance of shape, anchorage, and formation of nuclear lamina

12. Concept 6.7: Extracellular components and connections between cells help coordinate cellular activities Most cells synthesize and secrete materials that are external to the plasma membrane These extracellular structures include: – Cell walls of plants – The extracellular matrix (ECM) of animal cells – Intercellular junctions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

13. Cell Walls of Plants The cell wall is an extracellular structure that distinguishes plant cells from animal cells Prokaryotes, fungi, and some protists also have cell walls The cell wall protects the plant cell, maintains its shape, and prevents excessive uptake of water Plant cell walls are made of cellulose fibers embedded in other polysaccharides and protein Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

14. Plant cell walls may have multiple layers: – Primary cell wall: relatively thin and flexible – Middle lamella: thin layer between primary walls of adjacent cells – Secondary cell wall (in some cells): added between the plasma membrane and the primary cell wall Plasmodesmata are channels between adjacent plant cells Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

15. Fig. 6-28 Secondary cell wall Primary cell wall Middle lamella Central vacuole Cytosol Plasma membrane Plant cell walls Plasmodesmata 1 µm

16. The Extracellular Matrix (ECM) of Animal Cells Animal cells lack cell walls but are covered by an elaborate extracellular matrix (ECM) The ECM is made up of glycoproteins such as collagen, proteoglycans, and fibronectin ECM proteins bind to receptor proteins in the plasma membrane called integrins Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

17. Fig. 6-30 EXTRACELLULAR FLUID Collagen Fibronectin Plasma membrane Micro- filaments CYTOPLASM Integrins Proteoglycan complex Polysaccharide molecule Carbo- hydrates Core protein Proteoglycan molecule Proteoglycan complex

18. Functions of the ECM: – Support – Adhesion – Movement – Regulation Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

19. Intercellular Junctions Neighboring cells in tissues, organs, or organ systems often adhere, interact, and communicate through direct physical contact Intercellular junctions facilitate this contact There are several types of intercellular junctions – Plasmodesmata – Tight junctions – Desmosomes – Gap junctions Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

20. Plasmodesmata in Plant Cells Plasmodesmata are channels that perforate plant cell walls Through plasmodesmata, water and small solutes (and sometimes proteins and RNA) can pass from cell to cell Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

21. Fig. 6-31 Interior of cell 0.5 µm PlasmodesmataPlasma membranes Cell walls

22. Tight Junctions, Desmosomes, and Gap Junctions in Animal Cells At tight junctions, membranes of neighboring cells are pressed together, preventing leakage of extracellular fluid Desmosomes (anchoring junctions) fasten cells together into strong sheets Gap junctions (communicating junctions) provide cytoplasmic channels between adjacent cells Copyright © 2008 Pearson Education, Inc., publishing as Pearson Benjamin Cummings

23. Fig. 6-32 Tight junction 0.5 µm 1 µm Desmosome Gap junction Extracellular matrix 0.1 µm Plasma membranes of adjacent cells Space between cells Gap junctions Desmosome Intermediate filaments Tight junction Tight junctions prevent fluid from moving across a layer of cells

24. Extracellular Component Review Three functions of the cell wall? – Protection, maintain shape, and prevent excessive water uptake What are the intercellular junctions between plant cells? What can pass through them? – Plasmodesmata; cytosol passes through joining the internal chemical environments What are the intercellular junctions between animal cells? – Tight junctions, Gap junctions, & desmosomes Which animal junction is similar to plasmodesmata? – Gap Junctions