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Fig. S1 HUVECs HDFNs HUVECs HDFNs Transwell CM Relative total length ** * control 200ng/ml400ng/ml TIMP-1 100ng/ml * ** GFP WT Mutant TIMP-1 TIMP-1 **

Published byNathaniel Lewis Modified over 11 years ago

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Presentation on theme: "Fig. S1 HUVECs HDFNs HUVECs HDFNs Transwell CM Relative total length ** * control 200ng/ml400ng/ml TIMP-1 100ng/ml * ** GFP WT Mutant TIMP-1 TIMP-1 **"— Presentation transcript:

1 Fig. S1 HUVECs HDFNs HUVECs HDFNs Transwell CM Relative total length ** * control 200ng/ml400ng/ml TIMP-1 100ng/ml * ** GFP WT Mutant TIMP-1 TIMP-1 ** Relative total length Supplementary data, Liu et al., 2007 Control TIMP-1 MMP-2/9 inhibitor MMP-1 inhibitor ** Relative total length Control TIMP-1 TIMP-1 + MMP-9 MMP-9 ** Relative total length AB D E F Fig. S1. Quantitative analysis of vessel formation by relative total length. (A) Fibroblast- conditioned media promotes vessel formation. (B) Exogenous TIMP-1 enhances vessel formation. (C) Removal of TIMP-1 by siRNA from fibroblasts reduces conditioned- media induced vessel formation. (D) TIMP-1 promotes vessel formation in a MMP- dependent manner. (E) Inhibition of MMP-2/9 enhances vessel formation. (F) TIMP-1- enhanced vessel formation is abolished by active MMP-9. *P < 0.05, **P < 0.01 compared to respective control. C control TIMP-1 siRNA + TIMP-1 Fibroblast- conditioned media Con siRNA Relative total length **

2 Absorbance A 0h 24h 48h Con TIMP-1 B E D C 0h24h Fig. S2 Supplementary data, Liu et al., 2007 Fig. S2. TIMP-1 does not affect proliferation and migration of HUVECs. (A) HUVECs were cultured in a 96-well plate and incubated without (con) or with TIMP-1 (400ng/ml). At indicated time points cell number was determined using methylene blue assay. (B-E) HUVECs were plated in 24-well plate and migration was determined by wound healing assay. The monolayers were wounded and incubated without (con) (B and C) or with TIMP-1 (400ng/ml) (D and E).

3 Relative area con 0.01 0.03 0.1 0.3 1 3 10 MMP-2/9 inhibitor ( M) ** Fig. S3 Supplementary data, Liu et al., 2007 Fig. S3. Inhibition of MMP-2/9 enhances vessel formation. HUVECs were grown in three dimensional collagen gel for five days without (con) or with indicated concentrations of MMP-2/9 inhibitor. **P < 0.01 compared to control.

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