1. Oxalate (µmol/gFW) Itaconate (µmol/gFW) r=-0.702** Citrate (µmol/gFW) r=0.389 Isocitrate (µmol/gFW) Ascorbate (µmol/gFW) r=0.052 r=0.195 New Leaves 10 mM Itaconate 1 weeks10 mM Itaconate 2 weeks10 mM Itaconate 3 weeks Itaconate (µmol/gFW) Supplementary Data 1 Miyagi et al.

2. PC 1 (35.1 %) PC 2 (18.1 %) Stems : Organic acid : Amino acid : Phosphorylated PC 2 (18.1 %) PC 1 (35.1 %) Isocitrate Ornithine Ascorbate RuBP Coumarate 6PG Aconitate Gly FBP Asp Shikimate Pyruvate Succinate Citrate Cys Trp Ser Ala DHAP G6P Ru5P R5P Glu Cinnamate Citrulline GABA Tyr Phe Leu Lys Val Ile Asn Arg Thr Met Pro His 2OG PEP 3PGA Oxalate G1P Gln Malate Fumarate Isocitrate Ornithine Ascorbate RuBP Coumarate 6PG Aconitate Gly FBP Asp Shikimate Pyruvate Succinate Citrate Cys Trp Ser Ala DHAP G6P Ru5P R5P Glu Cinnamate Citrulline GABA Tyr Phe Leu Lys Val Ile Asn Arg Thr Met Pro His 2OG PEP 3PGA Oxalate G1P Gln Malate Fumarate Isocitrate Ornithine Ascorbate RuBP Coumarate 6PG Aconitate Gly FBP Asp Shikimate Pyruvate Succinate Citrate Cys Trp Ser Ala DHAP G6P Ru5P R5P Glu Cinnamate Citrulline GABA Tyr Phe Leu Lys Val Ile Asn Arg Thr Met Pro His 2OG PEP 3PGA Oxalate G1P Gln Malate Fumarate PC 1 loadings PC 2 loadings PC 3 loadings PC 3 (10.4%) Control 0 weekControl 1 weekControl 2 weeksControl 3 weeks 10 mM Itaconate 0 week10 mM Itaconate 1 weeks10 mM Itaconate 2 weeks10 mM Itaconate 3 weeks a b c d e f Supplementary Data 2 Miyagi et al. compound

3. Oxalate (µmol/gFW) Itaconate (µmol/gFW) r=-0.113 Citrate (µmol/gFW) r=-0.058 Isocitrate (µmol/gFW) Ascorbate (µmol/gFW) r=-0.488* r=-0.396 Stems 10 mM Itaconate 0 week10 mM Itaconate 1 weeks10 mM Itaconate 2 weeks10 mM Itaconate 3 weeks Itaconate (µmol/gFW) Supplementary Data 3 Miyagi et al.