1. Overview of the nutrition resources from the INMA cohort Valencia, Spain Jesús Vioque on behalf of INMA-Cohort Study Dpto. Salud Pública. Universidad Miguel Hernández

2. Cohorts from the INMA Study, Spain INMA project includes almost 4000 pregnant women from seven areas in Spain, and their newborns. The most recent cohorts used the same protocol (2004). >2500 mothers >2400 children 1y

3. Proyecto INMA “Infancia y Medio Ambiente”. (http://www.infanciaymedioambiente.org)http://www.infanciaymedioambiente.org INMA-Valencia Cohort 787 Births 732 (1y old) 591 (4y old)

4. 4 Dietary assesment FFQ -101 12 week FFQ -101 32 week FFQ -105 4y old PREGNANCY CHILDREN Validated Food Frequency Questionnaire (FFQ) -Biochemical validation: vitamin C, E, B12, folate and carotenoids Validated FFQ -Biochemical validation -24h-recalls (3) Daily Nutrient and Food Intakes (and supplements) r = 0.44 r = 0.42;

5. FFQs A semi-quantitative FFQ of 100 food items to assess the usual daily intake of foods and nutrients. Based on a previous FFQ validated in a adult population (Vioque, 2006) similar to the Harvard FFQ (Willett, 1985)

6. J Vioque6 Nutrientes del FFQ alfa-Carotene beta-Carotene Lutein+Zeaxanthin Lycopene Cryptoxanthin

7. Other variables for the INMA-Valencia cohort Variable name Numbers of participants Birth weightbw592 Gestational age (in weeks)ga591 Maternal BMImbmi592 Age (in months of adiposity assessment)age_1591 Heightheight591 Weightweight591 Waist circumferencewc591 DXA- assessed fat mass------- Bioelectrical impedance assessed fat massfm457 Age (in months) cardiovascular outcomes measuresage_2173 Diastolic blood pressureDbp420 Systolic blood pressureSbp420 Total cholesterolTchol173 HDLcHdl171 LDLcLdl171 TGtg172 Glucosegluc171 Insulin-------- CRPcrp151