1. Expression level ARR3 - + - + - + - + BA 30 120 min 200 150 100 50 0 ARR4 5000 4000 3000 2000 1000 0 - + - + - + - + BA 30 120 min ARR5 5000 4000 3000 2000 1000 0 - + - + - + - + BA 30 120 min ARR6 1500 1200 900 600 300 0 - + - + - + - + BA 30 120 min ARR16 400 300 200 100 0 - + - + - + - + BA 30 120 min Expression level ARR8 400 300 200 100 0 - + - + - + - + BA 30 120 min Expression level ARR7 20000 15000 10000 5000 0 - + - + - + - + BA 30 120 min ARR15 - + - + - + - + BA 30 120 min 250 200 150 100 50 0 ARR9 1200 900 600 300 0 - + - + - + - + BA 30 120 min Col-0 35S:ARR7 - + - + - + - + BA 30 120 min AHK1 1000 800 600 400 200 0 AHK4 500 400 300 200 100 0 600 - + - + - + - + BA 30 120 min Expression level Supplementary Figure 1 Analysis of A-type ARRs, AHK1, and AHK4 gene expression in response to cytokinins in wild type and 35S:ARR7. Seven-day-old light-grown seedlings of wild-type and 35S:ARR7 were either mock-treated or treated with 5 M BA for 30 min or 2 h, total RNA was isolated, and followed by the GeneChip experiments. Cytokinin-regulated A-type ARR and AHK genes that exhibit, on average, >2-fold change are presented. The expression levels (y axis) represent differential hybridizations between perfectly matched and mismatched oligodeoxyribonucleotides and average values of two independent biological experiments. Shaded and thick columns indicate wild-type and 35S:ARR7 transgenic plants, respectively

2. EXPA1 4000 3000 2000 1000 0 Expression level EXPA3 - + - + - + - + BA 30 120 min - + - + - + - + BA 30 120 min 400 200 0 600 EXPA10 800 600 400 200 0 - + - + - + - + BA 30 120 min EXPA15 1600 1200 800 400 0 - + - + - + - + BA 30 120 min EXPB3 1200 800 400 0 - + - + - + - + BA 30 120 min EXPA5 600 400 200 0 - + - + - + - + BA 30 120 min EXPA4 3000 2000 1000 0 - + - + - + - + BA 30 120 min Expression level EXPA6 - + - + - + - + BA 30 120 min 1200 800 400 0 1600 Expression level Supplementary Figure 2 Analysis of expansin gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1

3. LBD4 400 300 200 100 0 - + - + - + - + BA 30 120 min LBD3 400 300 200 100 0 - + - + - + - + BA 30 120 min Expression level Supplementary Figure 3 Analysis of LBD gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1

4. CKX2CKX3 CKX4 CKX5 CKXa 160 120 80 40 0 - + - + - + - + BA 30 120 min Expression level 120 80 40 0 - + - + - + - + BA 30 120 min 1200 800 400 0 - + - + - + - + BA 30 120 min 600 400 200 0 - + - + - + - + BA 30 120 min 500 400 200 0 - + - + - + - + BA 30 120 min Expression level Supplementary Figure 4 Analysis of cytokinin oxidase gene expression in response to cytokinins in wild- type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1

5. At1g03850 6000 4000 2000 0 At4g15680 Expression level At2g30540 400 300 200 100 0 - + - + - + - + BA 30 120 min At3g62930 400 300 200 100 0 Expression level - + - + - + - + BA 30 120 min - + - + - + - + BA 30 120 min - + - + - + - + BA 30 120 min 600 400 200 0 Supplementary Figure 5 Analysis of glutaredoxin gene expression in response to cytokinins in wild-type and 35S:ARR7. Experiments were done and analyzed as described in the legend of Supplementary Figure 1