1. BCG PPD-specific IFN-γ ELISpot Responses Weeks after immunization
Figure #1
BCG PPD-specific IFN-γ ELISpot Responses
rBCG
rBCG
rAd5
SFC/106 PBMC
Weeks after immunization
Median

2. SIV-specific IFN-γ ELISpot Responses
Figure #2
SIV-specific IFN-γ ELISpot Responses
a. Gag peptide pool stimulated
b. p11C peptide stimulated
rBCG
rBCG
rAd5
SFC/106 PBMC
c. Pol peptide pool stimulated
d. Env peptide pool stimulated
Weeks after immunization
Median

3. % Tetramer binding CD8+ T cells
Figure #3
SIV-specific CD8+ T-cell Responses after In Vitro Culture Stimulation of PBMC with p11C Peptide and Interleukin-2
% Tetramer binding CD8+ T cells
Monkey rBCG dose (cfu) Route

4. SIV Gag-specific IFN-γ ELISpot Responses by Dosage
Figure #4a
SIV Gag-specific IFN-γ ELISpot Responses by Dosage
2 weeks after rAd5
4 weeks after rAd5
SFC/106 PBMC
10 weeks after rAd5
21 weeks after rAd5

5. SIV Pol-specific IFNγ ELISpot Responses by Dosage
Figure #4b
SIV Pol-specific IFNγ ELISpot Responses by Dosage
2 weeks after rAd5
4 weeks after rAd5
SFC/106 PBMC
10 weeks after rAd5
21 weeks after rAd5

6. SIV Gag-specific IFNγ ELISpot Responses by Route
Figure #5a
SIV Gag-specific IFNγ ELISpot Responses by Route
2 weeks after rAd5
4 weeks after rAd5
SFC/106 PBMC
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.0079;
Dunn's Multiple Comparison
(adjusted for multiple comparison):
p<0.05 for BCG IV vs. Control: significant
p<0.05 for BCG ID vs. Control: significant
p>0.05 for all other group comparisons
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.0780;
Dunn's Multiple Comparison
(adjusted for multiple comparison):
p>0.05 between all groups

7. SIV Gag-specific IFN-γ ELISpot Responses by Route
Figure #5b
SIV Gag-specific IFN-γ ELISpot Responses by Route
10 weeks after rAd5
21 weeks after rAd5
SFC/106 PBMC
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.0406;
Dunn's Multiple Comparison
(adjusted for multiple comparison):
p>0.05 between all groups
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.0227;
Dunn's Multiple Comparison
(adjusted for multiple comparison):
p<0.05 for ID vs. Control,
p>0.05 for all other group comparisons

8. SIV Pol-specific IFN-γ ELISpot Responses by Route
Figure #5c
SIV Pol-specific IFN-γ ELISpot Responses by Route
2 weeks after rAd5
4 weeks after rAd5
SFC/106 PBMC
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.0146;
Dunn's Multiple Comparison
(adjusted for multiple comparison):
p<0.05 for BCG IV vs. Control: significant
p>0.05 for all other group comparisons
* Mann-Whitney Test
Kruskall-Wallis-Test: p= ;
Dunn's Multiple Comparison (adjusted for multiple comparison):
p>0.05 between all groups

9. SIV Pol-specific IFNγ ELISpot Responses by Route
Figure #5d
SIV Pol-specific IFNγ ELISpot Responses by Route
10 weeks after rAd5
21 weeks after rAd5
SFC/106 PBMC
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.3853;
Dunn's Multiple Comparison
(adjusted for multiple comparison):
p>0.05 between all groups
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.2565;
Dunn's Multiple Comparison
(adjusted for multiple comparison):
p>0.05 between all groups

10. SIV Gag-specific Tetramer Responses by Dosage
Figure #6
SIV Gag-specific Tetramer Responses by Dosage
2 weeks after rAd5
4 weeks after rAd5
% Tetramer binding CD8+ T cells
10 weeks after rAd5
21 weeks after rAd5

11. SIV Gag-specific p11C Tetramer Responses by Route
Figure #7a
SIV Gag-specific p11C Tetramer Responses by Route
2 weeks after rAd5
4 weeks after rAd5
% Tetramer binding CD8+ T cells
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.0132;
Dunn's Multiple Comparison (adjusted for multiple comparison):
p<0.05 for IV vs. Control: significant
p<0.05 for ID vs. Control: significant
p>0.05 for all other group comparisons
* Mann-Whitney Test
Kruskall-Wallis Test: p=0.1733;
Dunn's Multiple Comparison (adjusted for multiple comparison):
p>0.05 for all groups
Median ± Interquartile Range

12. SIV Gag-specific p11C Tetramer Responses by Route
Figure #7b
SIV Gag-specific p11C Tetramer Responses by Route
10 weeks after rAd5
21 weeks after rAd5
% Tetramer binding CD8+ T cells
* Mann-Whitney Test
Kruskall-Wallis Test : p=0.0869;
Dunn's Multiple Comparison (adjusted for multiple comparison):
p>0.05 for all groups
* Mann-Whitney Test
Kruskall-Wallis Test : p=0.0823;
Dunn's Multiple Comparison (adjusted for multiple comparison):
p>0.05 for all groups
Median ± Interquartile Range

13. SIV Gag-specific p11C Tetramer Responses by Route and Dosage
rBCG
rBCG
rAd5
Figure #8
% Tetramer binding CD8+ T cells
SIV Gag-specific p11C Tetramer Responses by Route and Dosage
Median
Weeks after immunization

14. SIV Pol-specific p68 Tetramer Responses
Figure #9
SIV Pol-specific p68 Tetramer Responses
rAd5
% Tetramer binding CD8+ T cells
Weeks after immunization
Median

15. ICS SIV Gag Peptide Pool Stimulation
Figure #10
ICS SIV Gag Peptide Pool Stimulation
3 weeks after rAd5
% Cytokine+ Gated on CD8+3+
% Cytokine+ Gated on CD4+3+
Median ± Interquartile Range

16. ICS p11C Peptide Stimulation
Figure #11a
ICS p11C Peptide Stimulation A)
rBCG IV
rBCG ID
rAd5
% cytokine+ B)
CD8+ T cells
IFN- + - + + - - +
IL-2 + + - + - + -
TNF-α + + + - + - -
Median ± Interquartile Range

17. ICS p11C Peptide Stimulation
Figure #11b
ICS p11C Peptide Stimulation
p11C
CD28+
Total cytokine
IFN-γ
TNF-α
IL-2
Median ± Interquartile Range