1. Biotechnology Development: Opportunities and Challenges
Dr Prakash Ghimire, Ph D
Associate Professor Microbiology
Tribhuvan University
Microbiologist, WHO-IPD/NPHL/EDCD-MoH

2. Ecological Zones and Development Regions of Nepal
Far-Western Region
Mid-Western Region
Western Region
Central Region
Eastern Region
Mountains (16 districts)
Hills (39 districts)
Terai (20 districts)

3. Biotechnology: Animal Pharmaceutical Plant Hybrids Diagnostic
Therapeutic
Vaccines
Plant
Agriculture - NARC
Food

4. Biotechnology: Post Human genome situation Cell biology Immunology
Proteomics & Genomics – two closely related area
Cell biology
Immunology
Antibodies
Polyclonal
Monoclonal
Molecular biology
Genomics
Proteomics

5. RONAST

6. RONAST ACTIVITIES: RESEARCH ACTIVITIES Biotechnology Programs -
Conservation of Medicinal and Aromatic Plants [Click Here]- Molecular Diagnostic of Citrus greening Disease –
Molecular Characterization of Medicinal plants  -Tea clones  -Bacillus thuringinsis –
Flora of Nepal Project Research on Organic Farming -Antagonistic Study of Ectomycorrhiza -Study of the role of Endomycorrhiza
Natural Products Research -Bioprospecting of Cordyceps sinensis-Biopesticides study program -Analytical services

7. Nepal Health Research Council
Supports health Research
Mostly in health system research
Negligible investment in Biotechnology

8. Medical Biotechnology In Nepal
Immunology:
Vaccine Production
Rabies Vaccine Production (Central Veterinary Research Laboratory), Tripureswor
Antibody Production
Everest Biotech
Founded by biochemists at Oxford University, based in Oxfordshire, UK with a production laboratory established in Kathmandu.
CDM TU- Non commercial/Academic Use
Antibody Production, Purification……..
National Public Health Laboratory
Health Services
Flow cytometric assay ( FACS)

9. Medical Biotechnology
Molecular Biology
DNA Sequencing – Proposed at National Forensic Laboratory, Khumaltar
PCR
RONAST- Plant molecular biology
CDMTU – Tropical diseases- Malaria, Leishmania etc.
National Public Health Laboratory, Teku- SARS, Malaria, etc.
VBDRTC, Hetauda- Malaria, Leishmania
Mycobacterial research Laboratory, Lele- Tuberculosis, Leprosy
Universal College/ RLAAB- Plant mol. Bio.
Veterinary Research Laboratory, Tripureswor-
Si-Gan Health Foundation, Maharajgunj
Real Time PCR ( Proposed)
National Public Health Laboratory, Teku
Avian Influenza diagnostic service
Cell Biology

10. Real-Time PCR

11. Challenges: Machines without consumables Academic & HMG Institutes
Training of Human Resources
Latest updates
Research Culture
Carrer prospects
Research Funding & Collaboration
NHRC ?
Big researches- Survey type, No Laboratory capacity development in Nepal
Availability of Consumables in local market
Mostly not available, If available, costs 3-5 times of original cost
Entrepreneurship
Market

12. Hope for Future New University programs as per market requirements
Medical technology Program
Medical Microbiology Program
Pharmaceutical Sciences Program
Biotechnology Program
Allied health Programs ( Radiology, Dentistry, Physiotherapy, Bio-medical Engineering, …….)
Availability of funding in post WTO scenario
Introduction of newer Pharmaceutical companies
Availability of trained /cost effective HRs
Worldwide market in post WTO situation
Coming up of new diagnostic companies
Positive Government Policy ?

13. A small truth to make our life 100% successful……
Formula for Success
A small truth to make our life 100% successful……
A B C D E F G H I J
K L M N O P Q R S
T U V W X Y Z

14. 47% 54% 70% 96% 98% LUCK 12+21+3+11 EFFORT 5+6+6+15+18+20 LOVE
54%
EFFORT
70%
KNOWLEDGE
96%
HARDWORK
98%
None of them makes 100%

15. Then what makes 100% Is it money? …No!!! Our Life 100% Successful
Is it leadership? …No!!!
Every problems has a solution, so perhaps
Change our “ATTITUDE”
It is ATTITUDE towards life and work that makes
Our Life 100% Successful

16. ATTITUDE
100%
Don’t you think so ?!?!?!?!

17. Thank You